Bioinformatics analysis performed on proteins toxicants that may be present in genetically enhanced alfalfa strengthens the safety of the crop as this shows the absence of biologically relevant structural similarities to any toxin observed for CP4 EPSPS protein.CP4 EPSPS is claimed to be easily digested in vitro and in mammalian digestive fluids upon ingestion. It is non toxic to acutely gavaged mice that refers to the safety of the protein (Harrison et al., 1996) and has no direct effect on metabolic pathways.Assessment of the CP4 EPSPS protein involved the use of an allergen sequence database which demonstrated absence of protein sequences which are potentially risky for humans.  The 8 contiguous amino acids indicative of immunologicaly allergenic sequence or epitopes were not detected.  This, then strengthens safety of the CP4 EPSPS protein in events J101 and J163.Degradation of CP4 EPSPS in simulated digestive fluids (SDS) and gastric fluids (SGF) was rapid with a half-life data of less than 15 seconds in the stomach and less than 10 minutes in the intestines.  The digestibility of CP4 EPSPS was evaluated by SDS-PAGE gel staining, Western blot analysis and enzymatic activity assay.  SDS-PAGE colloidal blue gel staining demonstrated that 98% of the protein was digested in SGF as confirmed by Western blot analysis.  This explains safe consumption of the crops harboring the protein.  The highest concentration of CP4 EPSPS protein (572 mg/kg) did not elicit any adverse effect in mice. No treatment related effects on body weight, food consumption, survival, clinical observations or gross pathology confirms non-toxicity of the protein.