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Organismos vivos modificados
(LMO)
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Insect-Resistant and Herbicide-Tolerant Maize
EN
4114
Sí
DP-ØØ4114-3
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Organización:Pioneer Hi-Bred International Inc. ()Sector privado (comercio e industria)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, Estados Unidos de AméricaTeléfono: +1 515 535-3200,Fax:Correo electrónico:Sitio web: www.pioneer.com/,
Maize (Zea mays) was modified for resistance to lepidoptera, including European corn borer (Ostrinia nubilalis), and coleoptera insect pests including corn rootworm pests, such as the western corn rootworm (Diabrotica virgifera virgifera), and tolerance to glufosinate-ammonium herbicide. To achieve resistance to lepidopteran insects, the maize expresses the cryIF gene from Bacillus thuringiensis. To achieve resistance to coleopteran insects, the maize expresses the cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis. The cry genes have a pore forming mode-of-action in the epithelial lining of feeding larvae; they produce a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve glufosinate tolerance, the maize expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
El término "organismo receptor" hace referencia a un organismo (modificado o no modificado) que fue objeto de una modificación genética, en tanto que "organismos parentales" hace referencia a los implicados en el cruzamiento o la fusión de células.
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BCH-ORGA-SCBD-246-6 Organismo Zea mays (Maize, Corn, MAIZE)Cultivos
Maize inbred line: PHWWE
EN
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DAS-59122-7 × DAS-Ø15Ø7-1 × MON-ØØ6Ø3-6 - Herculex XTRA™ Roundup Ready™ 2 maize| Pioneer Hi-Bred International Inc. | Resistencia a enfermedades y plagas (Insectos, Coleoptera (escarabajos), Lepidoptera (mariposas y polillas)), Resistencia a herbicidas (Glufosinato, Glifosato)
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DP-Ø32316-8 - Insect Resistant, Herbicide Tolerant Maize| Resistencia a enfermedades y plagas (Insectos, Lepidoptera (mariposas y polillas)), Resistencia a herbicidas (Glufosinato)
PHP27118
EN
- Transferencia de ADN mediada por Agrobacterium
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0.983 kb
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1.010 kb
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1.818 kb
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0.714 kb
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0.983 kb
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1.010 kb
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0.372 kb
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0.310 kb
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1.289 kb
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1.152 kb
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0.310 kb
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0.530 kb
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0.552 kb
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0.192 kb
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Algunos de estos elementos genéticos pueden estar presentes como fragmentos o formas truncadas. Por favor, véanse las notas a continuación, en los casos en los que corresponda.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promotor
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Secuencia de codificación de proteínas | Resistencia a enfermedades y plagas (Insectos, Lepidoptera (mariposas y polillas))
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BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminador
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BCH-GENE-SCBD-14994-9 Cry34Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Secuencia de codificación de proteínas | Resistencia a enfermedades y plagas (Insectos, Coleoptera (escarabajos))
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BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | Solanum tuberosum (Potato, SOLTU)Terminador
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BCH-GENE-SCBD-100368-6 Peroxidase gene promoter | Triticum aestivum (Wheat)Promotor
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BCH-GENE-SCBD-14995-8 Cry35Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Secuencia de codificación de proteínas | Resistencia a enfermedades y plagas (Insectos, Coleoptera (escarabajos))
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promotor
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Secuencia de codificación de proteínas | Resistencia a herbicidas (Glufosinato)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminador
Information on the inserted DNA sequences
The transforming plasmid for maize DP-ØØ4114-3 contained a transfer DNA region comprising four gene cassettes: three expressing insecticidal proteins, cryIF, cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis, and one cassette used for positive transformant selection expressing the herbicide tolerance protein phosphinothricin N-acetyltransferase (PAT).
(1) The cryIF gene is regulated by the ubiquitin gene promoter from Zea mays and the ORF25 polyA terminator sequence from Agrobacterium tumefaciens. An intron from Zea mays (intron 1) was included to enhance expression of the cryIF sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(2) The cry34Ab1 gene is regulated by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. The intron 1 from Z. mays was included to enhance expression of the cry34Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(3) The cry35Ab1 gene is regulated peroxidase gene promoter from Triticum aestivum by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. An intron from Z. mays was included to enhance expression of the cry35Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter.
(4) The pat is used as a selectable marker for transformants, and the gene is regulated by the Cauliflower mosaic virus 35S promoter and terminator.
The transforming plasmid for maize DP-ØØ4114-3 contained a transfer DNA region comprising four gene cassettes: three expressing insecticidal proteins, cryIF, cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis, and one cassette used for positive transformant selection expressing the herbicide tolerance protein phosphinothricin N-acetyltransferase (PAT).
(1) The cryIF gene is regulated by the ubiquitin gene promoter from Zea mays and the ORF25 polyA terminator sequence from Agrobacterium tumefaciens. An intron from Zea mays (intron 1) was included to enhance expression of the cryIF sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(2) The cry34Ab1 gene is regulated by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. The intron 1 from Z. mays was included to enhance expression of the cry34Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(3) The cry35Ab1 gene is regulated peroxidase gene promoter from Triticum aestivum by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. An intron from Z. mays was included to enhance expression of the cry35Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter.
(4) The pat is used as a selectable marker for transformants, and the gene is regulated by the Cauliflower mosaic virus 35S promoter and terminator.
Note
- The polyubiquitin gene promoter element is made up of the promoter region (900bp) and the 5’ untranslated region of the polyubiquitin gene (83 bp).
- Southern blot analyses indicated that a single, intact transformation cassette was inserted into the genome of 4114 maize with no integration of the backbone of plasmid PHP27118.
ES
- Alimento animal
- Alimentación
- DP-ØØ4114-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
ES
ES
- EUginius: 4114 [URL] [ English ]
- ISAAA GM Approval database: 4114 [URL] [ English ]
- Patent (2011). Maize event DP-004114-3 and methods for detection thereof [PDF] ( US20110154523A1 ) [ English ]
- OECD BioTrack database: DP-ØØ4114-3 [URL] [ English ]
- FAO GM Foods Platform: DP-ØØ4114-3 [URL] [ English ]
- FSANZ Safety Assessment (A1106). Food derived from Herbicide-tolerant & Insect-protected Corn Line 4114 [PDF] ( Food Standards Australia New Zealand ) [ English ]
- EFSA (2018). Assessment of maize 4114 [PDF] ( food and feed uses, Regulation (EC) No 1829/2003 (application EFSA-GMO-NL-2014-123) ) [ English ]
- USDA APHIS (2013). Pioneer Petition for the Determination of Nonregulated Status for Insect-Resistant and Herbicide-Tolerant 4114 Maize [PDF] ( 11_244_01p ) [ English ]
- USDA-APHIS: Final Environmental Assessment (DP-004114-3) [PDF] ( 11_244_01p ) [ English ]
- USDA-APHIS: Plant Pest Risk Assessment for Pioneer 4114 Maize [PDF] ( 11_244_01p ) [ English ]
- Food and Chemical Toxicology (2013). Thirteen week rodent feeding study with grain from molecular stacked trait lepidopteran and coleopteran protected (DP-ØØ4114-3) maize [PDF] [ English ]
- CFIA Decision (DD 2013-98). Determination of the safety of Pioneer Hi-Bred Production Ltd.'s corn (Zea mays L.) event 4114 [URL] [ English ]
| Tipo de registro | Campo | Registro(s) | |
|---|---|---|---|
| Organismos vivos modificados | Organismo receptor u organismos parentales | 10 | |
| Organismos vivos modificados | OVM relacionado(s) | 1 | |
| Decisión o cualquier otra comunicación de un país | Identificación del OVM | 12 | |
| Evaluación del riesgo generada por un proceso regulatorio | Organismo(s) vivo(s) modificado(s) | 12 | |
| Laboratorio dedicado a la detección e identificación de OVM | OVM detectable(s) por el laboratorio | 4 | |
