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Organismos vivos modificados (LMO)
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BCH-LMO-SCBD-14797-16   |   PDF   |   Imprimir   |  
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Decisiones sobre el OVM Evaluaciones del riesgo  
published: 05 jun. 2006 last updated: 13 jul. 2026
Identidad del organismo vivo modificado
En la imagen que se encuentra abajo se identifica el OVM mediante su identificador único, nombre comercial y enlace a esta página del CIISB. Haga clic en el enlace para descargar una imagen más grande en su computadora. Para obtener ayuda sobre cómo utilizarlo, vaya a las páginas de enlace rápido al OVM.
Agrisure™ CB/LL
EN
Bt11 (X4334CBR, X4734CBR)
Sí
SYN-BTØ11-1
Maize (Zea mays) was modified for resistance to the Lepidoptera insect European corn borer (Ostrinia nubilalis) and tolerance to glufosinate-ammonium herbicides. To achieve resistance to the European corn borer, the maize expresses cry1Ab gene from Bacillus thuringiensis subsp. kurstaki, which have a pore forming mode-of-action in the epithelial lining of feeding larvae; cry1Ab produces a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve resistance to glufosinate, the maize expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase encoding gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
EN
El término "organismo receptor" hace referencia a un organismo (modificado o no modificado) que fue objeto de una modificación genética, en tanto que "organismos parentales" hace referencia a los implicados en el cruzamiento o la fusión de células.
ES
  • BCH-LMO-SCBD-45400-3
    Bt-10 Maize
    | Resistencia a los antibióticos (Ampicilina), Resistencia a enfermedades y plagas (Insectos, Lepidoptera (mariposas y polillas), Taladro del maíz (Ostrinia nubilalis)), Resistencia a herbicidas (Glufosinato)
    Show detection method(s)
Características del proceso de modificación
pZO1502 Derived from pUC18
EN
  • Transferencia directa de ADN
 
0.510 kb
 
 
0.470 kb
 
 
1.850 kb
 
 
0.250 kb
 
 
0.420 kb
 
 
0.180 kb
 
 
0.550 kb
 
 
0.250 kb
 
Algunos de estos elementos genéticos pueden estar presentes como fragmentos o formas truncadas. Por favor, véanse las notas a continuación, en los casos en los que corresponda.
  • BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)
    Secuencia de codificación de proteínas | Resistencia a herbicidas (Glufosinato)
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Secuencia de codificación de proteínas | Resistencia a enfermedades y plagas (Insectos, Lepidoptera (mariposas y polillas))
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminador
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promotor
  • BCH-GENE-SCBD-103625-3 Alcohol dehydrogenase 1, intron 6 | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-103867-2 Alcohol dehydrogenase 1, intron 2 | Zea mays (Maize, Corn, MAIZE)
    Intron
Information on the inserted DNA sequences
The DNA insert contained two genetic cassettes expressing the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes. Both cassettes were introduced by particle acceleration (biolistic) transformation.

(1) The cry1Ab gene is under the control of the 35S Cauliflower mosaic virus promoter and the Agrobacterium tumefaciens nopaline synthase gene terminator. An alcohol dehydrogenase 1 intron (intron 6) from Zea mays was included to enhance expression of the cry1Ab sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.

(2) The pat gene is regulated by the 35S promoter from Cauliflower mosaic virus and the nopaline synthase gene terminator from Agrobacterium tumefaciens. The Zea mays alcohol dehydrogenase 1 intron (intron 2) from was also included to enhance expression of the phosphinothricin N-acetyltransferase sequence from Streptomyces viridochromogenes. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.

Note
  • The plasmid carrying the DNA transfer was digested by restriction enzyme NotI and only the fragments containing the two expression cassettes were inserted into the LM maize. The AMPr gene encoding ampicillin resistance and an origin of replication were not inserted.
  • Southern blot analysis confirmed the absence of unwanted DNA fragments in the transformant generations.
  • The CaMV promoter associated with cry1Ab, was isolated from the CM1841 strain of CaMV using DdeI restriction digestion. However, the DdeI sites were converted into SacI sites.
  • The CaMV promoter associated with pat was isolated from the Cabb-S strain of CaMV (AluI to DdeI fragment) and subsequently modified.
  • The cry1Ab coding sequence encodes a truncated version corresponding to the N-terminal 615 amino acids of the full length Cry1Ab protein.
  • The cloning of pat did not result in any amino acid sequence changes in the parental line.
  • The nos terminator corresponds to the 423 to 678 basepairs of the nopaline synthase gene in A. tumefaciens.
EN
Características del OVM
ES
  • Alimento animal
  • Alimentación
Información adicional
Information on the inserted DNA sequences
  • 35S promoters derived from cauliflower mosaic virus (CaMV) and 35S-1 originated from the CM1841 isolate of CaMV as a 500 Ddel to Ddel fragment, subsequently converted to Sacl sites and 35S-2 originated from the Cabb-S strain of CaMV as a n Alul to Ddel fragments (ca 425bp), whose ends were subsequently modified.
  • Introns derived from the maize alcohol dehydrogenase 1S gene and were used to enhance heterologous gene expression.
  • Btk gene which is an altered version of the full length cry1A(b) gene of Bacillus thuringiensis var kurstaki HD-1. The Btk was obtained as a 1.8kb Nco-Bg/ll fragment. The truncated Btk protein is identical to the N-terminal 615 amino acids of the native Btk protein of 1155 amino acids
  • pat gene (phosphinotricin acetyl transferase) cloned from the soil microorganism, Streptomyces viridochromogenes strain Tu494.  Alteration did not result in any amino acid sequence changes.
  • nos terminator consisting of 423-678 of the nopaline synthase gene of Agrobacterium tumefaciens plus added restriction sites.

    Vector information
    Plasmid pZO1502 is the vector used for the transformation of Bt maize.  This is a derivative of plasmid pUC18. The plasmid has a molecular weight of 2.7 kb and contains the following sequences:
    - the prokaryotic gene bla (also called ampR) under a procaryotic promoter encoding β-lactamase, which confers resistance to ampicillin; it is used as a bacterial selectable marker;  
    - the gene lac Z, encoding a portion of a β-galactosidase, this gene is not functional; 
    - the pUC origin of replication derived from the plasmid pBR 322 carrying a mutation.
EN
Registros que hacen referencia a este documento Mostrar en la búsqueda
Tipo de registro Campo Registro(s)
Organismos vivos modificados Organismo receptor u organismos parentales 68
Organismos vivos modificados OVM relacionado(s) 1
Decisión o cualquier otra comunicación de un país Identificación del OVM 42
Evaluación del riesgo generada por un proceso regulatorio Organismo(s) vivo(s) modificado(s) 40
Laboratorio dedicado a la detección e identificación de OVM OVM detectable(s) por el laboratorio 33

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