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Organismos vivos modificados
(LMO)
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Herculex™ I maize
EN
TC1507
Sí
DAS-Ø15Ø7-1
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Organización:Dow AgroSciences ()Teléfono:Fax:Correo electrónico:Sitio web: http://www.dowagro.com/homepage/index.htm,
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Organización:Pioneer Hi-Bred International Inc. ()Sector privado (comercio e industria)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, Estados Unidos de AméricaTeléfono: +1 515 535-3200,Fax:Correo electrónico:Sitio web: www.pioneer.com/,
Maize (Zea mays) was modified for resistance to the lepidopteran European corn borer (Ostrinia nubilalis) and tolerance to herbicide glufosinate ammonium. To achieve lepidopteran resistance, the modified corn Bacillus thuringiensis expresses the Cry1F protein (delta-endotoxin/crystal protein),which has a pore forming mode of action in the epithelial lines of the feeding insect larvae. To achieve tolerance to the herbicide glufinosinate, the modified corn expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which acetylates the glufosinate ammonium herbicide to form a non-toxic compound, preventing the inhibition of glutamine synthetase and thus preserving ammonia levels and nitrogen metabolism in the plant.
El término "organismo receptor" hace referencia a un organismo (modificado o no modificado) que fue objeto de una modificación genética, en tanto que "organismos parentales" hace referencia a los implicados en el cruzamiento o la fusión de células.
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BCH-ORGA-SCBD-246-6 Organismo Zea mays (Maize, Corn, MAIZE)Cultivos
ES
PHI8999A derived from plasmid PHP8999
EN
- Biolística / Pistola de partículas
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0.980 kb
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1.000 kb
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1.820 kb
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0.720 kb
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0.550 kb
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0.550 kb
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0.200 kb
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Algunos de estos elementos genéticos pueden estar presentes como fragmentos o formas truncadas. Por favor, véanse las notas a continuación, en los casos en los que corresponda.
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BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Secuencia de codificación de proteínas | Resistencia a enfermedades y plagas (Insectos, Lepidoptera (mariposas y polillas))
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Secuencia de codificación de proteínas | Resistencia a herbicidas (Glufosinato)
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promotor
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BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminador
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promotor
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminador
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
The DNA insert from maize DAS-Ø15Ø7-1 contained two genetic cassettes expressing the cry1F gene from Bacillus thuringiensis and the phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes.
(1) The cry1F gene regulated by the promoter and first exon and intron of the maize ubiquitin gene. The 3' terminator sequence used was the 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) (see footnote*). Due to the constitutive nature of the promoter, high levels of expression in all plant tissues are expected from this cassette.
(2) The pat coding sequence was under control of the Cauliflower mosaic virus 35S promoter and terminator. Due to the viral nature of the promoter, high levels of transcriptional expression in all plant tissues are expected from this genetic cassette.
(1) The cry1F gene regulated by the promoter and first exon and intron of the maize ubiquitin gene. The 3' terminator sequence used was the 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) (see footnote*). Due to the constitutive nature of the promoter, high levels of expression in all plant tissues are expected from this cassette.
(2) The pat coding sequence was under control of the Cauliflower mosaic virus 35S promoter and terminator. Due to the viral nature of the promoter, high levels of transcriptional expression in all plant tissues are expected from this genetic cassette.
Note
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat genes are identical to those in the original plasmid. The proteins produced in the modified plants are the ones intended, including a leucine residue (replacing a phenylalanine) at position 604 (of 605 amino acids in total) of Cry1F. This modification was introduced to create a specific restriction site for cloning purposes.
- Molecular analyses of the transformed plant show that the event TC1507 contains one site of integration of the introduced DNA which includes a full-length of the DNA fragment used for transformation (i.e. the ~6235 bp of DNA cassette containing the cry1F and pat genes) and an additional copy of the cry1F gene lacking the majority of the associated ubiquitin regulatory sequences.
- Southern analysis using a cry1F probe carried out by the European Food Safety Authority (EFSA; see document below) also revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F fragment of 335bp, which is located at the 5’ end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat coding sequence, the maize ubiquitin promoter and the terminator from Agrobacterium tumefaciens. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.
Footnote *: The EFSA document indicates that the 3’ sequence from the A. tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
ES
- Biocombustible
- Alimento animal
- Alimentación
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database [ English ]
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
ES
The transformation event TC1507 is the genetic modification present in the commercial maize variety Fenaltec 22, the first locally developed modified crop released for commercial cultivation in Colombia and the first off-patent (generic) GM maize commercialized in Latin America. Developed through a collaboration between the National Federation of Cereal, Legumes, and Soybean Growers (FENALCE) and the Plant Genetic Engineering Group from National University of Colombia, it incorporates the previously approved TC1507 event into a Colombian-adapted maize variety. Following the expiration or absence of applicable patent protections, existing biotechnology events can be legally integrated into locally adapted crops, reducing development costs and dependence on proprietary technologies. Fenaltec 22 received authorization for commercial cultivation in Colombia in 2019, followed by approvals for food and feed use, marking an important milestone for public-sector agricultural biotechnology and national seed development in the region (Sánchez-Betancourt et al., 2024). See attached for publication.
- EUginius: DAS1507 [URL] [ English ]
- OECD BioTrack database: 1507 [URL] [ English ]
- ISAAA GM Approval database: TC1507 [URL] [ English ]
- FAO GM Approval database: DAS-Ø15Ø7-1 [URL] [ English ]
- BATS (2003), Genetically Modified (GM) Crops: Molecular and Regulatory details, version 2 [PDF] [ English ]
- EFSA (2005). Opinion of the Scientific Panel on Genetically Modified Organisms on placing on the market of insect-tolerant genetically modified maize 1507, for food use (EFSA-GMO-NL-2004-02) [PDF] [ English ]
- Dow AgroSciences LLC (2000): DAS-Ø15Ø7-1 [PDF] [ English ]
- FSANZ: Assessment Report A446 (corn line 1507) [PDF] [ English ]
- Canadian Food Inspection Agency (2002). DD2002-41 - Determination of the Safety of Corn (Zea mays L.) Line 1507 [URL] [ English ]
- Crop Breed. Appl. Biotechnol. (2024). First Latin American off-patent corn event - Fenaltec 22 [PDF] ( Crop Breeding and Applied Biotechnology, Brazilian Society of Plant Breeding ) [ English ]
- Crop Breed. Appl. Biotechnol. (2024). First Latin American off-patent corn event - Fenaltec 22 [PDF] ( Crop Breeding and Applied Biotechnology; Brazilian Society of Plant Breeding; Sánchez-Betancourt et al., 2024 ) [ English ]
| Tipo de registro | Campo | Registro(s) | |
|---|---|---|---|
| Organismos vivos modificados | Organismo receptor u organismos parentales | 120 | |
| Organismos vivos modificados | OVM relacionado(s) | 3 | |
| Decisión o cualquier otra comunicación de un país | Identificación del OVM | 76 | |
| Evaluación del riesgo generada por un proceso regulatorio | Organismo(s) vivo(s) modificado(s) | 90 | |
| Laboratorio dedicado a la detección e identificación de OVM | OVM detectable(s) por el laboratorio | 30 | |
