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Organisme vivant modifié
(LMO)
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Bollgard™ cotton
EN
MON531
Oui
MON-ØØ531-6
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Organisation :Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, États-Unis d'AmériqueTéléphone : + 1 314 694-1000,Télécopieur : +1 314 694-3080,E-mail :Site Web : http://www.monsanto.com,
The cotton (Gossypium hirsutum) was modified for insect resistance through the expression of Bacillus thuringiensis crystal protein (delta-endotoxin) Cry1Ac, which has a pore-forming mode of action in the midgut of feeding insects. The Cry1Ac protein confers resistance to Lepidoptera insects, such as cotton bollworm, tobacco budworm and pink bollworm. The modified cotton additionally contains an antibiotic resistance cassette (Escherichia coli neomycin phosphotransferase II) for kanamycin selection during transformation of the plants.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
Cultivar Coker 312
EN
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MON-89924-2 - Bollgard™ cotton| Monsanto | Résistance aux antibiotiques (Kanamycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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MON-ØØ757-7 - Bollgard™ cotton| Monsanto | Résistance aux antibiotiques (Kanamycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
PV-GHBK04
EN
- Transgénèse par agrobacterium
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Kanamycine)
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)Terminateur
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Streptomycine)
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
The modified cotton contains two gene cassettes: Bacillus thuringiensis cry1Ac and Escherichia coli neomycin phosphotransferase II (nptII).
The cry1Ac coding sequence is under control of Cauliflower mosaic virus (CaMV) 35S enhanced promoter and Glycine max α' subunit of β-conglycinin terminator. See notes below about the partial cry1Ac cassette and antibiotic resistance gene aad.
The nptII coding sequence is under control of a CaMV 35S promoter and and Agrobacterium tumefaciens nopaline synthase terminator.
Note:
The cry1Ac coding sequence is under control of Cauliflower mosaic virus (CaMV) 35S enhanced promoter and Glycine max α' subunit of β-conglycinin terminator. See notes below about the partial cry1Ac cassette and antibiotic resistance gene aad.
The nptII coding sequence is under control of a CaMV 35S promoter and and Agrobacterium tumefaciens nopaline synthase terminator.
Note:
- Due to the constitutive nature of the viral promoters, high levels of expression are expected in all plant tissues.
- The cry1Ac coding sequence was codon-optimized for expression in plants. The codon optimization resulted in a single amino acid change (leucine substituted for serine) at position 766 (L766S). The sequence was originally sourced from B. thuringiensis subsp. kurstaki HD-73.
- Southern blot analysis indicated that the LMO contains a single active copy and a partial inactive copy of the cry1Ac gene both of which are linked.
- The plasmid also contains the antibiotic resistance 3"(9)-O-aminoglycoside adenylyltransferase (aad) gene. This gene confers resistance to the antibiotics spectinomycin and streptomycin, and facilitated the selection of bacteria containing the plasmid in the initial steps of transforming the cotton tissue. The aad gene is under the control of a bacterial promoter. Studies using ELISA testing have indicated that there there is no detectable expression of the aad gene in the modified line.
FR
- Autres (Industrial use)
- MON-ØØ531-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-ØØ531-6 - CropLife International Detection Methods Database ( CropLife ) [ English ]
FR
The cry1Ac gene found in the modified cotton is almost identical to that found in nature and in commercial Bt spray formulations.
Cry proteins, of which Cry1Ac is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis.
Cry1Ac only acts on the larvae of lepidopteran insects (moths and butterflies), due to the presence of specific binding sites (receptors) in the target insects, which are not found in other types of insects. There are no receptors for delta-endotoxins of Bacillus thuringiensis on the surface of mammalian intestinal cells and therefore, livestock animals and humans are not susceptible to these proteins.
Cry proteins, of which Cry1Ac is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis.
Cry1Ac only acts on the larvae of lepidopteran insects (moths and butterflies), due to the presence of specific binding sites (receptors) in the target insects, which are not found in other types of insects. There are no receptors for delta-endotoxins of Bacillus thuringiensis on the surface of mammalian intestinal cells and therefore, livestock animals and humans are not susceptible to these proteins.
- OECD UID Database [ English ]
- MON-ØØ531-6 APHIS.pdf [ English ]
- MON-ØØ531-6 Monsanto.pdf [ English ]
- EUginius - MON531 [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Organisme vivant modifié | Organisme récepteur ou organismes parentaux | 3 | |
| Organisme vivant modifié | OVM connexe(s) | 3 | |
| Décision de pays ou tout autre communication | Identification de l'OVM | 26 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 18 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 16 | |
