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Organisme vivant modifié
(LMO)
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Bollgard®, Roundup Ready® and LibertyLink® Cotton
EN
MON531 × MON1445 × LLCotton25
Oui
MON-Ø531-6 x MON-Ø1445-2 x ACS-GHØØ1-3
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Organisation :GENSUS S.A ()Secteur privé (affaires et industrie)San Vladimiro 3056, San Isidro (B1642GMB) Buenos Aires, ArgentinaSan Isidro, Buenos Aires
B1642GMB, ArgentineTéléphone :Télécopieur :E-mail : consultas@gensus.com.ar,Site Web : https://gensus.com.ar/,
The cotton (Gossypium hirsutum) was produced through cross breeding of modified parental varieties MON531 × MON1445 and ACS-GHØØ1-3. For resistance to lepidopteran insect pests, the cotton expresses Bacillus thuringiensis Cry1Ac, an insecticidal crystal protein active against susceptible Lepidoptera including cotton bollworm, pink bollworm and tobacco budworm. The protein acts through disruption of the insect midgut following ingestion. For tolerance to glyphosate, the cotton expresses the CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS), which has reduced sensitivity to glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway. For tolerance to glufosinate ammonium herbicides, the cotton expresses phosphinothricin N-acetyltransferase (PAT), derived from Streptomyces hygroscopicus, which inactivates glufosinate through acetylation.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-LMO-SCBD-14775-17 Organisme vivant modifié MON-ØØ531-6 - Bollgard™ cottonMonsanto | Résistance aux antibiotiques (Kanamycine, Néomycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-LMO-SCBD-14851-8 Organisme vivant modifié ACS-GHØØ1-3 - Liberty Link™ cottonBayer CropScience | Résistance aux herbicides (Glufosinate)
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BCH-LMO-SCBD-14880-14 Organisme vivant modifié MON-Ø1445-2 - Roundup Ready™ cottonMonsanto | Résistance aux antibiotiques (Kanamycine, Streptomycine), Résistance aux herbicides (Glyphosate)
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
FR
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MON-ØØ531-6 × MON-Ø1445-2 - Roundup Ready™ Bollgard™ Cotton| Monsanto | Résistance aux antibiotiques (Kanamycine, Streptomycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites)), Résistance aux herbicides (Glyphosate)
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ACS-GHØØ1-3 × MON-15985-7 - Liberty Link™ Bollgard II™ cotton| Bayer CropScience | Résistance aux antibiotiques (Streptomycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites)), Résistance aux herbicides (Glufosinate)
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BCS-GHØØ2-5 × ACS-GHØØ1-3 - GlyTol™ Liberty Link™ cotton| Résistance aux herbicides (Glufosinate, Glyphosate)
PV-GHBK04, pGSV71 and PV-GHGT07
EN
- Transgénèse par agrobacterium
- Croisement
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)Terminateur
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Streptomycine)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Kanamycine)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoteur
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminateur
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
DNA insert from MON-ØØ531-6
The transforming plasmid PV-GHBK04 contained two gene cassettes expressing Cry1Ac and NPTII.
-The cry1Ac coding sequence is under control of Cauliflower mosaic virus (CaMV) 35S enhanced promoter and Glycine max α' subunit of β-conglycinin terminator. See notes below about the partial cry1Ac cassette and antibiotic resistance gene aad.
-The nptII coding sequence is under control of a CaMV 35S promoter and and Agrobacterium tumefaciens nopaline synthase terminator.
Note:
-The cry1Ac coding sequence is under control of Cauliflower mosaic virus (CaMV) 35S enhanced promoter and Glycine max α' subunit of β-conglycinin terminator. See notes below about the partial cry1Ac cassette and antibiotic resistance gene aad.
-The nptII coding sequence is under control of a CaMV 35S promoter and and Agrobacterium tumefaciens nopaline synthase terminator.
Note:
- Due to the constitutive nature of the viral promoters, high levels of expression are expected in all plant tissues.
- The cry1Ac coding sequence was codon-optimized for expression in plants. The codon optimization resulted in a single amino acid change (leucine substituted for serine) at position 766 (L766S). The sequence was originally sourced from B. thuringiensis subsp. kurstaki HD-73.
- Southern blot analysis indicated that the LMO contains a single active copy and a partial inactive copy of the cry1Ac gene both of which are linked.
- The plasmid also contains the antibiotic resistance 3"(9)-O-aminoglycoside adenylyltransferase (aad) gene. This gene confers resistance to the antibiotics spectinomycin and streptomycin, and facilitated the selection of bacteria containing the plasmid in the initial steps of transforming the cotton tissue. The aad gene is under the control of a bacterial promoter. Studies using ELISA testing have indicated that there there is no detectable expression of the aad gene in the modified line.
DNA insert from MON-Ø1445-2
The transforming plasmid PV-GHGT07 contained gene cassettes expressing CP4 EPSPS and NPTII.
-The cp4 epsps coding sequence is under control of the Modified Figwort Virus 35S promoter and the Pisum sativum rbcS E9 terminator. The coding sequence includes the Arabidopsis thaliana EPSPS chloroplast transit peptide (CTP2), which directs the expressed protein to the chloroplast.
-The cp4 epsps coding sequence is under control of the Modified Figwort Virus 35S promoter and the Pisum sativum rbcS E9 terminator. The coding sequence includes the Arabidopsis thaliana EPSPS chloroplast transit peptide (CTP2), which directs the expressed protein to the chloroplast.
-The nptII coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase terminator.
Note:
Due to the constitutive nature of the viral promoters, expression is expected in most plant tissues.
- The CP4 EPSPS protein is targeted to the chloroplast through the Arabidopsis thaliana EPSPS chloroplast transit peptide (CTP2). Following transport into the chloroplast, the transit peptide is cleaved, producing the mature CP4 EPSPS protein.
- Southern blot analysis indicated that the modified line contains a single insertion event comprising approximately 6.1 kb of DNA. Molecular characterization demonstrated that the integrated DNA includes the cp4 epsps, nptII and aad sequences, together with a truncated oriV sequence.
- The gox gene cassette originally present in the transformation plasmid PV-GHGT07 was not integrated into the cotton genome.
- The plasmid also contains the antibiotic resistance 3''(9)-O-aminoglycoside adenylyltransferase (aad) gene. This gene confers resistance to the antibiotics spectinomycin and streptomycin and facilitated the selection of bacteria containing the plasmid during vector construction. The aad gene is under the control of a bacterial promoter and is not expected to be expressed in plant tissues. No detectable expression of the AAD protein was observed in the modified line.
DNA insert from ACS-GHØØ1-3
The transforming plasmid pGSV71 contained one gene cassettes expressing the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar). The bar coding sequence is under control of a Cauliflower mosaic virus 35S promoter and an Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the viral promoter, high levels of transcription are expected from this gene cassette.
Note:
Note:
- The initial two codons of the N-terminal of the bar coding sequence were synthetically modified to plant-preferred codons.
- Southern blot analysis indicated that a single intact copy of the T-DNA was integrated into the cotton genome and no vector backbone sequences were present.
FR
- Nourriture pour les animaux
- Fibre / textile
- Nourriture
The presence of each individual event can be detected by PCR using oligonucleotide sequences specific to each event. In this case, the method is based on detecting the simultaneous presence of each individual event from DNA extracted from a single biological sample.
FR
- Argentina Resolución 132/2025 [ Spanish ]
- ISAAA database - MON-ØØ531-6 x MON-Ø1445-2 x ACS-GHØØ1-3 [ English ]
- OECD Biotrack database - MON-ØØ531-6xMON-Ø1445-2xACS-GHØØ1-3 [ English ]
- EUginius - MON531 [ English ]
- EUginius - MON1445 [ English ]
- EUginius - LLcotton25 [ English ]
