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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato modified for increased stomata density
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Lines: 2, 6, 12
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Organization:Max Planck Institute of Molecular Plant Physiology (MPIMP)Academic or research institute, Max-Planck-Institut für Molekulare PflanzenphysiologieAm Mühlenberg 1Potsdam,
14476 , GermanyPhone: +49 331 567 - 80 ,Fax:Email: contact@mpimp-golm.mpg.de,
Transgenic potatoes expressing an RNAi construct against the endogenous StSDD1 gene. The resulting plant exhibits an increase in stomata density by up to 25%. Thereby these plants have enhanced transpiration activity, decreased leaf roll at high light intensities, and increased tuber yield.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Cultivar/breeding line: Désirée
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Potato modified for decreased Stomata Density| Resistance to antibiotics (Hygromycin), Tolerance to abiotic stress (Drought)
pBinAR-StSDD1 (Derived from pBIN19)
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-48460-3 Stomatal density and distribution 1 gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | Tolerance to abiotic stress (Drought)
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
Transgenic potatoes pBINAR-StSDD1 contain the StSDD1 RNAi construct consisting of the CaMV 35S promoter, a fragment of the endogenous StSDD1 gene in sense and antisense orientation separated by a part of the multiple cloning site of the vector pCR2.1-TOPO and the ocs terminator that mediates increased formation of stomata in leaves. These potatoes further encode an nptII cassette as a selection marker system that confers tolerance to aminoglycoside antibiotics (kanamycin, neomycin, geneticin). Here, the nptII gene is put under the control of the nos promoter and –terminator.
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BCH-GENE-SCBD-48460-3 Stomatal density and distribution 1 gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | Tolerance to abiotic stress (Drought)
In order to increase guard cell density, the expression of the potato's own SDD1 protein was reduced in these potato plants with the aid of an RNAi construct (pBinAR-StSDD1). Using the RNAi construct, the RNA of a part of the SDD1 gene is transcribed in sense and antisense orientation so that sense and antisense RNA are separated by a sufficiently long spacing sequence. The sense and antisense sections of the RNAi form a RNA doublestrand. The RNA double strand is recognized by specific enzymes in the plant cell and broken down into small fragments. These RNA fragments adhere to the mRNA of the endogenous SDD1 gene and activate the degradation of the mRNA.
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- Research
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- Binary Agrobacterium vectors for plant transformation. [ English ]
- On Defining T-DNA [ English ]
- Summary Notification - European Commission Joint Notification Center [ English ]
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