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Living Modified Organism (LMO)
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BCH-LMO-SCBD-101898-7   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 10 Jun 2011 last updated: 13 Jul 2026
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-protected and herbicide-tolerant cotton
EN
GHB119
Yes
BCS-GHØØ5-8
The cotton (Gossypium hirsutum) was modified for lepidopteran insect pests and glufosinate-ammonium tolerance. To achieve resistance against Lepidoptera, the cotton expresses the cry2Ae gene from Bacillus thuringiensis, which produces a crystal delta-endotoxin Cry2Ae with a pore-forming mode of action in the midgut epithelium of feeding larvae. To achieve glufosinate tolerance, the cotton expresses the the bialaphos resistance gene from Streptomyces hygroscopicus to produce phosphinothricin N-acetyltransferase, which inactivates the herbicide by acetylating L-glufosinate ammonium. 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar Coker 312
EN
Characteristics of the modification process
pTEM12 derived from pGSC1700
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-101900-6 CsVMV promoter | Cassava vein mosaic virus (Cassava vein mosaic virus, CVMV, CsVMV)
    Promoter
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
    Leader
  • BCH-GENE-SCBD-101902-5 Ribulose-1,5-bisphosphate carboxylase small subunit transit peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-101895-8 Cry2Ae | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), Fall armyworm (Spodoptera frugiperda))
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
Information about the inserted DNA sequences
The transforming plasmid for cotton BCS-GHØØ5-8 contains two gene cassettes: Streptomyces hygroscopicus phosphinothricin N-acetyltransferase encoding gene, also known as the bialaphos resistance gene (bar), and Bacillus thuringiensis cry2Ae gene.

(1) The bar coding sequence is under control of a Cassava vein mosaic virus promoter and an Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the promoters, high levels of transcription from the bar gene cassettes are expected.

(2) The cry2Ae coding sequence is under control of a Cauliflower mosaic virus 35S promoter and terminator. Due to the constitutive nature of the promoters, high levels of transcription from the cry2Ae gene cassettes are expected.

Note
  • Southern Blot analysis indicated that a single copy of the insert is present in the GHB119 parental line and a configuration that correspond to that in the original vector. No insertion of plasmid backbone sequences were detected, however three ORF were present at the junctions of the integration site but do not have the required regulatory elements for functionality. 
EN
LMO characteristics
EN
  • Feed
  • Fiber/textile
  • Food (Industrial)
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism or Parental Organisms 10
Country's Decision or any other Communication LMO identification 12
Risk Assessment generated by a regulatory process Living modified organism(s) 11
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 4
Risk Assessment generated by an independent or non-regulatory process Living modified organism(s) 1

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