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Living Modified Organism (LMO)
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Plum Tree modified for resistance to Plum pox virus
EN
C5
Yes
ARS-PLMC5-6
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Person:Dr. Ralf ScorzaResearch Horticulturist and Lead Scientist, Agricultural Research Service2217 WILTSHIRE ROAD Kearneysville, WV 25430Kearneysville, West Virginia
25430, United States of AmericaPhone: 304 725 3451 322,Fax:Email: Ralph.Scorza@ars.usda.gov,Website:Related OrganizationUnited States Department of Agriculture ()2217 WILTSHIRE ROAD Kearneysville, WV 25430Kearneysville, West Virginia
25430, United States of AmericaPhone: 304 725 3451 322,Fax:Email: Ralph.Scorza@ars.usda.gov,Website:
Plum tree Stanley clone C5 was modified to contain the coat protein gene of Plum pox virus. The insertion of the construct changes the properties of the recipient’s organism so that plum obtains Plum pox virus coat protein gene into its genetic information. This gene encodes the synthesis of a pox virus coat protein which in turn confers resistance against the PPV virus.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-104307-2 Organism Prunus domestica (Plum tree, Plum)Trees
Cultivar: Stanley; Clone: C5
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pGA482GG/PPVCP-33
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- Agrobacterium-mediated DNA transfer
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0.250 kb
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0.790 kb
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1.200 kb
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0.850 kb
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1.810 kb
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0.250 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-104309-1 PPV coat protein | Plum pox virus (PPV, Sharka )Protein coding sequence | Resistance to diseases and pests (Viruses)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
Clone C5 appeared to have at least two inserts with one being a possible rearrangement of the PPV-CP gene. This plant had significantly lower amounts of RNA transcript and no detectable protein.
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EN
- Food
- Research
EN
EN
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