Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism (LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant Herbicide tolerant Maize
EN
Event 32
Yes
DAS-59132-8
-
Organization:Dow AgroSciences ()Phone:Fax:Email:
-
Organization:Pioneer Hi-Bred International Inc. ()Private sector (business and industry)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, United States of AmericaPhone: +1 515 535-3200,Fax:Email:Website: www.pioneer.com/,
The transgenic maize line DAS-59132-7 was genetically modified to resist Coleopteran insects (e.g. western corn rootworm, northern corn rootworm and Mexican corn rootworm) and to tolerate herbicides containing glufosinate ammonium.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Maize line: Hi-II
EN
-
DAS-59122-7 - Herculex™ RW Rootworm Protection maize| Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Coleoptera (beetles)), Resistance to herbicides (Glufosinate)
PHP17662
EN
- Agrobacterium-mediated DNA transfer
|
1.300 kb
|
|
1.150 kb
|
|
0.320 kb
|
|
0.550 kb
|
|
0.550 kb
|
|
0.200 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
-
BCH-GENE-SCBD-14994-9 Cry34Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
-
BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | Solanum tuberosum (Potato, SOLTU)Terminator
-
BCH-GENE-SCBD-100368-6 Peroxidase gene promoter | Triticum aestivum (Wheat)Promoter
-
BCH-GENE-SCBD-14995-8 Cry35Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
The cry34Ab1 and cry35Ab1 were cloned from Bacillus thuringiensis strain PS149B1 and the coding sequence of both genes has been adapted to the codon usage in maize as to achieve expression in the maize plant.
Southern blot analysis indicated that a single insert of the transformation cassette was introduced into the modified maize. A 450bp truncation of the 5' end was detected, which resulted in a 200bp truncation of the Ubiquitin gene promoter. The absence of the spectinomycin and tetracycline resistance genes from the vector backbone was shown by hybridizing the Southern blots with spc and tet probes.
EN
Southern blot analysis indicated that a single insert of the transformation cassette was introduced into the modified maize. A 450bp truncation of the 5' end was detected, which resulted in a 200bp truncation of the Ubiquitin gene promoter. The absence of the spectinomycin and tetracycline resistance genes from the vector backbone was shown by hybridizing the Southern blots with spc and tet probes.
EN
- Food
- Feed
EN
EN
Loading...
