Genetic characterization studies demonstrated that MZHG0JG corn contains, at a single locus within the corn genome, a single copy of each of the following functional elements: mepsps-02, pat-09, FMV-05 enhancer, 35S-05 enhancer, OTP-02 transit peptide, Ubi158-02 promoter, TMV-03 enhancer, Ubi158-02 terminator, 35S-19 promoter, and NOS-05-01 terminator. It does not contain any extraneous DNA fragments of these functional elements elsewhere in the MZHG0JG corn genome, and it does not contain the plasmid backbone sequence from transformation plasmid pSYN18857.

Nucleotide sequence analysis determined that the MZHG0JG insert consists of the intact T-DNA region of the pSYN18857 plasmid vector. The results of the Southern blot analyses are consistent with the results of the nucleotide sequence analysis.

Sequence analysis of the MZHG0JG insertion site demonstrated that 22 bp from the corn genomic sequence were deleted during the integration of the MZHG0JG insert, and 43 bp of DNA were inserted into the integration site; a 4-bp DNA sequence was present at the junction between the MZHG0JG insert and the 5ʹ flanking region and a 39-bp DNA sequence was present at the junction between the MZHG0JG insert and the 3ʹ flanking region. BLASTN analyses comparing the corn genomic sequence flanking the MZHG0JG insert with sequences in public databases indicated that the insert does not disrupt any known endogenous corn gene.