Oilseed rape modified for an altered fatty acid metabolism in the seeds | BCH-LMO-SCBD-110915 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 12 Sep 2016
Living Modified Organism identity
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Oilseed rape modified for an altered fatty acid metabolism in the seeds
EN
Independent lines transformed with one of pHS124, pHS126, or pHS127
No
Subgenic domains of an oleate desaturase gene (E12) from Brassica napus in expresses in the anti-sense orientation leading to the inhibition of the desaturation of oleic acid (C18:1) to linoleic acid (C18:2) in the genetically modified oilseed rape plants.

As a result, the oleic acid content is increased in the seeds, while the linoleic and linolenic acid content is reduced.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • BCH-LMO-SCBD-101385-4
    Brassica napus modified for altered fatty acid metabolism
    | Bundesanstalt für Züchtungsforschung an Kulturpflanzen(BAZ) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-110921-1
    Oilseed rape modified for an altered fatty acid metabolism in the seeds
    | Deutsche Saatveredelung AG(DSV) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-110914-1
    Oilseed rape modified for an altered fatty acid metabolism in the seeds
    | Deutsche Saatveredelung AG(DSV) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-110916-1
    Oilseed rape modified for an altered fatty acid metabolism in the seeds
    | Deutsche Saatveredelung AG(DSV) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-110917-1
    Oilseed rape modified for an altered fatty acid metabolism in the seeds
    | Deutsche Saatveredelung AG(DSV) | Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-110918-1
    Oilseed rape modified for an altered fatty acid metabolism in the seeds
    | Deutsche Saatveredelung AG(DSV) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-110919-1
    Oilseed rape modified for an altered fatty acid metabolism in the seeds
    | Deutsche Saatveredelung AG(DSV) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-110920-1
    Oilseed rape modified for an altered fatty acid metabolism in the seeds
    | Deutsche Saatveredelung AG(DSV) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
Characteristics of the modification process
pHS124, pHS126, or pHS127
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-103920-2 Acyl-acyl carrier protein thioesterase promoter | Cuphea lanceolata (Cigar Flower)
    Promoter
  • BCH-GENE-SCBD-110907-1 Fatty acid deasturase 2 gene | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
  • BCH-GENE-SCBD-103921-2 Acyl-acyl carrier protein thioesterase terminator | Cuphea lanceolata (Cigar Flower)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
Three constructs containing 400 - 700 bp fragments the oleate desaturase gene from Brassica napus in an antisense orientation were produced.

All three fragments are expressed under the control of the promoter and the terminator signal of the Acyl-[ACP] thioesterase gene from Cuphea lanceolata.

Each transgenic line originated from a transformation with one of the three constructs.

The neomycin phosphotransferase gene (nptII) from the Tn5 transposon expressed under the control of the 35S promoter and termination signal of the Cauliflower Mosaic Virus (CaMV) was used as a selection marker.

The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 8

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