DAS-Ø15Ø7-1 × DAS-59122-7 × MON-ØØ81Ø-6 - Insect resistant, herbicide tolerant maize | BCH-LMO-SCBD-112033 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 15 Jun 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant, herbicide tolerant maize
EN
TC1507 x 59122 x MON810
Yes
DAS-Ø15Ø7-1 × DAS-59122-7 × MON-ØØ81Ø-6
The stacked maize line DAS-Ø15Ø7-1 x DAS-59122-7 x MON-ØØ81Ø-6 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of Cry1F, PAT, Cry34Ab1, Cry35Ab1 and Cry1Ab genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)
    Crops
  • BCH-LMO-SCBD-14841-13 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maize
    Dow AgroSciences, Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-15165-13 Living Modified Organism DAS-59122-7 - Herculex™ RW Rootworm Protection maize
    Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Coleoptera (beetles)), Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-14750-19 Living Modified Organism MON-ØØ81Ø-6 - YieldGard™ maize
    Monsanto | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
EN
Characteristics of the modification process
PHI8999A, PHP17662, PV-ZMBK07 and PV-ZMGT10
EN
  • Cross breeding
 
1.980 kb
 
 
1.820 kb
 
 
0.720 kb
 
 
0.610 kb
 
 
0.800 kb
 
 
3.460 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-14994-9 Cry34Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | Solanum tuberosum (Potato, SOLTU)
    Terminator
  • BCH-GENE-SCBD-100368-6 Peroxidase gene promoter | Triticum aestivum (Wheat)
    Promoter
  • BCH-GENE-SCBD-14995-8 Cry35Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Coleoptera (beetles))
  • BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome. MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from TC1507 vector PHI8999A
The TC1507 line contained the Cry1F and PAT coding sequences and regulatory elements. The Cry1F gene produces insecticidal crystal protein that confers resistance against lepidopteran pests and the PAT gene produces Phosphinothricin N-acetyltransferase which confers tolerance to the glufosinate herbicide.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
EN
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