Based on molecular characterization of GR2E rice, one copy of the pSYN12424 T-DNA was introduced at a single site within the rice genome and stably inherited over multiple generations as a single genetic locus according to Mendelian rules of inheritance. In addition, nucleotide sequencing of the entire inserted DNA, including portions of the 5’ and 3’ flanking rice genomic sequence, confirmed that the T-DNA had been inserted without modifications, deletions, or rearrangements, except for small truncations at the 5’ and 3’ termini of 23 bp and 11 bp, respectively. There were also no new novel open reading frames created as a consequence of the DNA insertion that would have the potential to encode a protein with any significant amino acid sequence similarity to known and putative toxins or allergens.