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Living Modified Organism (LMO)
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Provitamin A biofortified riceEN
- Organization: International Rice Research Institute () | BCH-CON-SCBD-112996-1International Rice Research Institute ()Private sector (business and industry)
Rice event GR2E (IR-ØØGR2E-5) was developed through the use of recombinant-DNA techniques to express elevated levels of provitamin A (mainly β-carotene) in the rice endosperm, which is converted in the body to vitamin A.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
japonica rice cultivar Kaybonnet
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | (Maize, Corn)Promoter
BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | (Maize, Corn)Intron
BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | (Bacteria)Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene TerminatorTerminator
BCH-GENE-SCBD-112995-1 Glutelin gene promoter | (Rice)Promoter
BCH-GENE-SCBD-103620-2 Phytoene Synthase 1 gene | (Maize, Corn)Protein coding sequence | Changes in quality and/or metabolite content (Vitamins)
BCH-GENE-SCBD-103616-4 rbcS Transit Peptide | (garden pea)Transit signal
BCH-GENE-SCBD-103621-3 Phytoene Desaturase geneProtein coding sequence | Changes in quality and/or metabolite content (Vitamins)
Based on molecular characterization of GR2E rice, one copy of the pSYN12424 T-DNA was introduced at a single site within the rice genome and stably inherited over multiple generations as a single genetic locus according to Mendelian rules of inheritance. In addition, nucleotide sequencing of the entire inserted DNA, including portions of the 5’ and 3’ flanking rice genomic sequence, confirmed that the T-DNA had been inserted without modifications, deletions, or rearrangements, except for small truncations at the 5’ and 3’ termini of 23 bp and 11 bp, respectively. There were also no new novel open reading frames created as a consequence of the DNA insertion that would have the potential to encode a protein with any significant amino acid sequence similarity to known and putative toxins or allergens.