The modified Anopheles coluzzii (Ac(DSM)2) was produced by crossing modified Anopheles gambiae (Ag(DSM)2) with wild-type An. coluzzii. The modified mosquitoes are male-sterile and express a fluorescent marker. For sterility, the modified mosquito expresses a homing endonuclease gene (Physarum polycephalum intron-encoded endonuclease; I-PpoI) in testes tissues, which cuts a ribosomal DNA sequence on the X chromosome during male spermatogenesis. However, all spermatozoa, irrespectively of the inheritance of the gene cassettes, carried a substantial amount of I-PpoI protein. Thus, upon mating and fertilization of the egg, the maternal X chromosome is also cut due to the activity of I-PpoI delivered from the sperm, leading to developmental arrest of embryos and no viable progeny. Thus, the destruction of the X chromosomes renders the male mosquitoes sexually sterile and unable to father viable progeny as a result of the I-PpoI activity on the X chromosome. In addition, the I-PpoI endonuclease is fused to Aequorea victoria enhanced green fluorescent protein, which was used to verify expression of I-PoI in the testes. The modified mosquito also expresses Discosoma sp. red fluorescent protein (DsRed2), which acts as a marker, being expressed in the eyes of the modified mosquitoes. The strain is maintained in the laboratory by crossing Ac(DSM)2 females, identified by red fluorescent eyes with wild-type males.