Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant eucalpytus
EN
751K032
No
-
Organization:Suzano S.A. ()Escritório São Paulo Avenida Brigadeiro Faria Lima, 1355São Paulo, São Paulo
, BrazilPhone: +55 800 022 1727, +55 11 3503 9000,Fax:Email: suzanoresponde@suzano.com.br,Website: https://www.suzano.com.br/,
The eucalyptus (Eucalyptus globulus) was modified for herbicide tolerance through the expression of Rhizobium radiobacter (Agrobacterium tumefaciens) 5-enolpyruvylshikimate-3-phosphate synthase (epsps). The bacterial enzyme does not bind the herbicide glyphosate with high affinity (unlike the endogenous EPSPS protein) and thus allows for the continued biosynthesis of aromatic amino acids through the shikimate pathway during glyphosate applications. In addition to the epsps cassette, the eucalyptus also expresses Escherichia coli neomycin phosphotransferase II, which was used for kanamycin selection during transformation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-102151-5 Organism Eucalyptus globulus (Eucalyptus, Tasmanian Blue Gum, Southern Blue Gum, Blue gum)Trees
EN
pBI121
EN
- Agrobacterium-mediated DNA transfer
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
-
BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
The eucalyptus contains two gene cassettes: Rhizobium radiobacter 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and Escherichia coli neomycin phosphotransferase (nptII).
The epsps coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and R. radiobacter nopaline synthase terminator.
The nptII coding sequence is under control of a CaMV 35 promoter and terminator.
Due to the constitutive nature of the CaMV promoter, expression of EPSPS and NPTII are expected to occur at high levels.
The epsps coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and R. radiobacter nopaline synthase terminator.
The nptII coding sequence is under control of a CaMV 35 promoter and terminator.
Due to the constitutive nature of the CaMV promoter, expression of EPSPS and NPTII are expected to occur at high levels.
EN
- Timber
EN
EN
- Alerta Diário - Diário Oficial da União - Seção 1 - Atos Normativos [ Portuguese ]
- CropLife Brasil - 751K032 Eucalipto [ Portuguese ]
