BCS-GH811-4 × ACS-GHØØ1-3 × MON-887Ø1-3 - Herbicide-tolerant cotton | BCH-LMO-SCBD-262939 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 20 Jan 2023
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant cotton
EN
GHB811 × LLcotton25 × MON88701
Yes
BCS-GH811-4 × ACS-GHØØ1-3 × MON-887Ø1-3
  • - Person: Bayer CropScience Bayer CropScience | BCH-CON-US-7091-2
    Person:
    Bayer CropScience Bayer CropScience
    Phone:
    Fax:
    Email:
    Website:
    Related Organization
    Bayer CropScience ()
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The modified cotton (Gossypium hirsutum) was produced through the cross breeding of modified parental lines to confer tolerance to multiple herbicides. To confer resistance to isoxaflutole, the cotton expresses Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD) W336, which contains a point mutation that confers reduced binding affinity to HPPD inhibitors. For tolerance to glyphosate, the cotton expresses the Zea mays double mutant 5-enol pyruvylshikimate-3-phosphate synthase, which has a reduced binding affinity to the herbicide.  For tolerance to dicamba, the cotton expresses Stenotrophomonas maltophilia dicamba monooxygenase, which inactivates the herbicide via oxidative demethylation. For tolerance to glufosinate, the cotton expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivate the herbicide via acetylation. 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)
    Crops
  • BCH-LMO-SCBD-113966-4 Living Modified Organism BCS-GH811-4 - Herbicide-tolerant cotton
    Bayer CropScience | Resistance to herbicides (Glyphosate)
  • BCH-LMO-SCBD-14851-8 Living Modified Organism ACS-GHØØ1-3 - Liberty Link™ cotton
    Bayer CropScience | Resistance to herbicides (Glufosinate)
  • BCH-LMO-SCBD-105602-3 Living Modified Organism MON-887Ø1-3 - Dicamba- and Glufosinate-tolerant cotton
    Monsanto | Resistance to herbicides (Glufosinate)
EN
Characteristics of the modification process
pTSIH09; pGSV71; PV-GHHT6997
EN
  • Cross breeding
 
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from GHB811 (BCS-GH811-4) vector pTSIH09
The GHB811 parental genome contains two gene cassettes: Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (hppd) and Zea mays 5-enolpyruvylshikimate-3-phosphate synthase (2mepsps).

The hppd coding sequence is under control of a Cassava vein mosaic virus promoter and Arabidopsis thaliana histone H4 terminator. A synthetic optimized (N-terminal) transit peptide was included to target the translated HPPD protein to the chloroplast. High levels of expression are expected due to the nature of the viral promoter. The 2mepsps coding sequence is under control of an A. thaliana histone H4 promoter and 3'untranslated region. An A. thaliana histone H3 intron was included to enhance the expression of 2mepsps. A synthetic optimized (N-terminal) transit peptide is also present to direct the protein to the chloroplasts.

Note:
Molecular characterization of the parental event indicated that a single copy of the T-DNA was inserted into the genome without the integration of vector backbone sequences.

DNA insert from LLcotton25 (ACS-GHØØ1-3) vector pGSV71
The modified cotton contains a single gene cassette: Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar). The bar coding sequence is under control of a Cauliflower mosaic virus 35S promoter and an Agrobacterium tumefaciens nopaline synthase terminator. Due to the constitutive nature of the viral promoter, high levels of transcription are expected from this gene cassette.

Note:
  • The initial two codons of the N-terminal of the bar coding sequence were synthetically modified to plant-preferred codons.
  • Southern blot analysis indicated that a single intact copy of the T-DNA was integrated into the cotton genome and no vector backbone sequences were present.

DNA insert from MON87701 (MON-887Ø1-3) vector PV-GHHT6997
The modified cotton contains two gene cassettes: Stenotrophomonas maltophilia dicamba monooxygenase (dmo) and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).

The dmo coding sequence is under control of a Peanut chlorotic streak virus promoter and Gossypium barbadense E6 terminator. A Tobacco etch virus 5' untranslated region was added between the promoter and dmo coding sequence to enhance transcription of dmo. The bar coding sequence is under control of a Cauliflower mosaic virus 35S enhanced promoter and an Agrobacterium tumefaciens nopaline synthases terminator. A Petunia hybrida heat shock protein 70 leader was added between the viral promoter and the bar coding sequence to enhance expression of bar.

Note:
Southern Blot and sequence analysis indicated that the transformation cassette was inserted into the MON88701 genome at a single intact locus containing all the genetic elements without the integration of vector backbone sequences.

For more information, kindly refer to the parental records.
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LMO characteristics
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  • Feed
  • Fiber/textile
Additional Information
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