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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant maize
EN
ACS-ZMØØ3-2 × DAS-4Ø278-9
Yes
ACS-ZMØØ3-2 × DAS-4Ø278-9
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Organization:Pioneer Hi-Bred International Inc. ()Private sector (business and industry)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, United States of AmericaPhone: +1 515 535-3200,Fax:Email:Website: www.pioneer.com/,
The maize was produced through the cross breeding of modified parental varieties for tolerance to multiple herbicides. For tolerance to glufinosate, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates phosphinothricin (the active ingredient in glufosinate ammonium herbicides) through acetylation. Additionally, the modified maize expresses Sphingobium herbicidovorans aryloxyalkanoate dioxygenase, which cleaves 2,4-dichlorophenoxyacetic acid into non-herbicidal dichlorophenol and glyoxylate, as well as inactivates aryloxyphenoxypropionate herbicides (acetyl‐CoA carboxylase inhibitors).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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BCH-LMO-SCBD-14767-14 Living Modified Organism ACS-ZMØØ3-2 - Liberty Link™ maizeBayer CropScience | Resistance to antibiotics (Ampicillin), Resistance to herbicides (Glufosinate)
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BCH-LMO-SCBD-104814-2 Living Modified Organism DAS-4Ø278-9 - Enlist™ MaizeDow AgroSciences GmbH | Resistance to herbicides, Tolerance to 2,4-Dichlorophenoxyacetic acid, Tolerance to aryloxyphenoxypropionate
EN
pDH51; pDAS1740
EN
- Cross breeding
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0.860 kb
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2.630 kb
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0.520 kb
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0.530 kb
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0.200 kb
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1.166 kb
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1.991 kb
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0.891 kb
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0.365 kb
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1.166 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14975-5 Beta-lactamase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Ampicillin)
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BCH-GENE-SCBD-101411-3 pUC origin of replicationPlasmid Vector
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-104795-4 RB7 matrix attachment region | Nicotiana tabacum (Tobacco, TOBAC )Enhancer
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-104812-3 Aryloxyalkanoate dioxygenase gene | Sphingobium herbicidovorans (SPHHE)Protein coding sequence | Resistance to herbicides,Tolerance to 2,4-Dichlorophenoxyacetic acid,Tolerance to aryloxyphenoxypropionate
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BCH-GENE-SCBD-104813-4 Per5 3' Untranslated Region | Zea mays (Maize, Corn, MAIZE)Terminator
DNA insert from T25 (ACS-ZMØØ3-2) vector pDH51
The parental genome contains a Streptomyces viridochromogene phosphinothricin N-acetyltransferase gene (pat) gene cassette. Transcription of pat is under control of a Cauliflower mosaic virus 35S promoter and 35S terminator. Due to the constitutive nature of the viral promoter, expression of pat is therefore expected to occur at elevated levels in all tissues.
The inserted T-DNA additionally contains a fragment of the bacterial Escherichia coli beta-lactamase gene and the plasmid vector origin of replication (for bacterial cells). These are not anticipated to be active in plant cells.
Note:
The parental genome contains a Streptomyces viridochromogene phosphinothricin N-acetyltransferase gene (pat) gene cassette. Transcription of pat is under control of a Cauliflower mosaic virus 35S promoter and 35S terminator. Due to the constitutive nature of the viral promoter, expression of pat is therefore expected to occur at elevated levels in all tissues.
The inserted T-DNA additionally contains a fragment of the bacterial Escherichia coli beta-lactamase gene and the plasmid vector origin of replication (for bacterial cells). These are not anticipated to be active in plant cells.
Note:
- The pat coding sequence was codon optimized for expression in plants without resulting in amino acid changes in the translated protein.
- A single pat cassette is present in the inserted DNA.
DNA insert from DAS40278 (DAS-4Ø278-9) vector pDAS1740
The parental LMO was generated using the Whiskers-mediated transformation method and contains a Sphingobium herbicidovorans aryloxyalkanoate dioxygenase-1 (aad-1) gene cassette.
The aad-1 coding sequence is under the control of a Zea mays ubiquitin gene promoter and Z. mays root preferential cationic peroxidase terminator. Due to the constitutive nature of the ubiquitin promoter, elevated levels of transcription of this gene cassette are expected to occur.
Note:
- The aad-1 coding sequence was optimized for expression in the plant.
- Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus.
- No integration of the vector backbone occurred.
Kindly refer to the parental LMO records for more information
EN
- Feed
- Food
- ACS-ZMØØ3-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- DAS-4Ø278-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- ACS-ZMØØ3-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- DAS-4Ø278-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- ACS-ZMØØ3-2 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
EN
- EUginius - T25 x DAS40278 [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
