The maize (Zea mays) was produced through cross‑breeding of modified parental varieties MON-89Ø34-3, MON-87411-9, DAS-Ø15Ø7-1, DAS-59122-7, and DAS-4Ø278-9.
Maize MON-89Ø34-3 was modified for resistance against lepidopteran pests such as fall armyworm (Spodoptera sp.), black cutworm (Agrotis ipsilon), European corn borer (Ostrinia nubilalis) and the corn earworm (Helicoverpa zea). To achieve Lepidoptera resistance, the maize expresses two Bacillus thuringiensis toxins encoded by the genes cry1A.105 and cry2Ab2. 

Maize MON-87411-9 was modified for resistance to coleopteran insect pests and tolerance to glyphosate herbicide. To achieve Coleoptera resistance, the maize expresses the gene, the maize expresses inverted repeat sequences of the Snf7 gene designed to matching the sequence of Western corn rootworm (Diabrotica virgifera virgifera), which trigger the rootworm's RNA interference pathway response to block replication, which results in the directed degradation of the endogenous Snf7 protein and cause death upon consumption of the LM maize. The maize also expresses the cry3Bb1 gene from Bacillus thuringiensis subsp. kumamotoensis, which have a pore forming mode-of-action in the epithelial lining of feeding larvae. To achieve glyphosate tolerance, the maize expresses enzyme 5-enolpyruvylshikimate-3-phosphate synthase, encoded by the cp4 epsps gene from Agrobacterium tumefaciens strain CP4, which has reduced binding affinity for glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway in the presence of the herbicide.
Maize DAS-Ø15Ø7-1 was modified for resistance to the lepidopteran European corn borer (Ostrinia nubilalis) and tolerance to herbicide glufosinate ammonium. To achieve lepidopteran resistance, the modified corn Bacillus thuringiensis expresses the Cry1F protein (delta-endotoxin/crystal protein),which has a pore forming mode of action in the epithelial lines of the feeding insect larvae. To achieve tolerance to the herbicide glufinosinate, the modified corn expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which acetylates the glufosinate ammonium herbicide to form a non-toxic compound, preventing the inhibition of glutamine synthetase and thus preserving ammonia levels and nitrogen metabolism in the plant.
Maize DAS-59122-7 was modified for resistance against coleopteran insect pests, such as Western corn rootworm (Diabrotica virgifera virgifera), Northern corn rootworm (Diabrotica barberi) and Mexican corn rootworm (Diabrotica virgifera zeae), and for tolerance to herbicides containing glufosinate-ammonium. To achieve Coleoptera resistance, the maize expresses the cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis. The cry genes have a pore forming mode-of-action in the epithelial lining of feeding larvae; they produce a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve glufosinate tolerance, the maize expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.

Maize DAS-4Ø278-9 was modified for tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate (AOPP) acetyl coenzyme A carboxylase (ACCase) inhibitors. To achieve herbicide tolerance, the maize expresses an optimised coding sequence for aryloxyalkanoate dioxygenase from Sphingobium herbicidovoran.