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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
published: 05 Mar 2009 last updated: 15 Aug 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato modified for resistance to Phytophthora infestans
EN
278 lines
No
The Rpi-blb1 and Rpi-blb2 genes inserted in these genetically modified potatoes should confer increased resistance to Phytophthora infestans. Rpi-blb1 and Rpi-blb2 code for proteins of the NBS-LRR (nucleotide binding site-leucine rich repeat) type.

The ahas gene inserted in the potato plants imparts tolerance to the herbicidal active substance Imazamox to the shoots during the selection process in cell culture.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
Vector VCPMA19 derived from pPZP200
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-41318-4 Phytophthora infestans Resistance gene 2 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)
    Protein coding sequence | Resistance to diseases and pests (Phytophthora infestans resistance)
  • BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
  • BCH-GENE-SCBD-41317-6 Phytophthora infestans Resistance gene 1 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)
    Protein coding sequence | Resistance to diseases and pests (Fungi)
  • BCH-GENE-SCBD-103775-2 Phytophthora infestans Resistance gene 2 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)
    Promoter
  • BCH-GENE-SCBD-103776-2 Phytophthora infestans Resistance gene 2 Terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)
    Terminator
  • BCH-GENE-SCBD-103777-3 Phytophthora infestans Resistance gene 1 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)
    Promoter
  • BCH-GENE-SCBD-103778-3 Phytophthora infestans Resistance gene 1 terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
EN
LMO characteristics
EN
  • Food
Detection method(s)
Observed gene expression in the transgenic plants

In the genetically modified lines a low level of expression of both Rpi-blb1 and Rpi-blb2 has been demonstrated by real-time PCR analysis in leaves, stems, tubers and roots. In flowers a low expression of Rpi-blb2 can be detected, while Rpi-blb1 is expressed at even lower levels or not at all.
The ahas gene is controlled by the nos promoter, which is known to result in low expression levels in all parts of the plant. Expression of the ahas gene in the genetically modified potato material has been demonstrated in tissue culture by survival on media containing Imazamox.
EN
Additional Information
Vector information
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant Mol. Biol., 25, 989-994
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication LMO identification 2
Risk Assessment generated by a regulatory process Living modified organism(s) 4
Living Modified Organism Related LMO(s) 1