Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Potato modified for resistance to Phytophthora infestans
EN
278 lines
No
-
Person:BASF Plant Science GmbHCarl-Bosch-Str. 38Ludwigshafen,
67056 , GermanyPhone: +49 621 60-0,Fax: +49 621 60-42525,Email: global.info@basf.com,
The Rpi-blb1 and Rpi-blb2 genes inserted in these genetically modified potatoes should confer increased resistance to Phytophthora infestans. Rpi-blb1 and Rpi-blb2 code for proteins of the NBS-LRR (nucleotide binding site-leucine rich repeat) type.
The ahas gene inserted in the potato plants imparts tolerance to the herbicidal active substance Imazamox to the shoots during the selection process in cell culture.
EN
The ahas gene inserted in the potato plants imparts tolerance to the herbicidal active substance Imazamox to the shoots during the selection process in cell culture.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
EN
Vector VCPMA19 derived from pPZP200
EN
- Agrobacterium-mediated DNA transfer
|
0.288 kb
|
|
2.013 kb
|
|
0.253 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-41318-4 Phytophthora infestans Resistance gene 2 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Protein coding sequence | Resistance to diseases and pests (Phytophthora infestans resistance)
-
BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
-
BCH-GENE-SCBD-41317-6 Phytophthora infestans Resistance gene 1 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Protein coding sequence | Resistance to diseases and pests (Fungi)
-
BCH-GENE-SCBD-103775-2 Phytophthora infestans Resistance gene 2 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Promoter
-
BCH-GENE-SCBD-103776-2 Phytophthora infestans Resistance gene 2 Terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Terminator
-
BCH-GENE-SCBD-103777-3 Phytophthora infestans Resistance gene 1 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Promoter
-
BCH-GENE-SCBD-103778-3 Phytophthora infestans Resistance gene 1 terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Terminator
-
BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
-
BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
EN
EN
- Food
Observed gene expression in the transgenic plants
In the genetically modified lines a low level of expression of both Rpi-blb1 and Rpi-blb2 has been demonstrated by real-time PCR analysis in leaves, stems, tubers and roots. In flowers a low expression of Rpi-blb2 can be detected, while Rpi-blb1 is expressed at even lower levels or not at all.
The ahas gene is controlled by the nos promoter, which is known to result in low expression levels in all parts of the plant. Expression of the ahas gene in the genetically modified potato material has been demonstrated in tissue culture by survival on media containing Imazamox.
EN
In the genetically modified lines a low level of expression of both Rpi-blb1 and Rpi-blb2 has been demonstrated by real-time PCR analysis in leaves, stems, tubers and roots. In flowers a low expression of Rpi-blb2 can be detected, while Rpi-blb1 is expressed at even lower levels or not at all.
The ahas gene is controlled by the nos promoter, which is known to result in low expression levels in all parts of the plant. Expression of the ahas gene in the genetically modified potato material has been demonstrated in tissue culture by survival on media containing Imazamox.
Vector information
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant Mol. Biol., 25, 989-994
EN
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant Mol. Biol., 25, 989-994
- 07-r42-01-app-a1.pdf [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 2 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 4 | |
| Living Modified Organism | Related LMO(s) | 1 | |