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Living Modified Organism (LMO)
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Fortuna potato
EN
PH05-026-0048
Yes
BPS-PHØ48-1
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Person:BASF Plant Science GmbHCarl-Bosch-Str. 38Ludwigshafen,
67056 , GermanyPhone: +49 621 60-0,Fax: +49 621 60-42525,Email: global.info@basf.com,
The potato was modified for resistance to Phytophthora infestans through the expression of the resistance (R) genes Rpi-blb1 and Rpi-blb2 from a wild relative Solanum bulbocastaneum. The R genes encode nucleotide-binding site-leucine rich repeat type proteins and play a role in host defence against the pathogenic fungus.
Additionally, a selectable marker, Arabidopsis thaliana acetohydroxyacid synthase, was included for imidazolinone selection during transformation. The enzyme carries a point mutation, S653N, which confers herbicide tolerance.
EN
Additionally, a selectable marker, Arabidopsis thaliana acetohydroxyacid synthase, was included for imidazolinone selection during transformation. The enzyme carries a point mutation, S653N, which confers herbicide tolerance.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
EN
Vector VCPMA16 derived from pPZP200
EN
- Agrobacterium-mediated DNA transfer
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0.288 kb
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2.013 kb
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0.253 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-41318-3 Phytophthora infestans Resistance gene 2 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Protein coding sequence | Resistance to diseases and pests (Phytophthora infestans resistance)
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BCH-GENE-SCBD-41317-5 Phytophthora infestans Resistance gene 1 | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103775-1 Phytophthora infestans Resistance gene 2 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Promoter
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BCH-GENE-SCBD-103776-1 Phytophthora infestans Resistance gene 2 Terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Terminator
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BCH-GENE-SCBD-103777-2 Phytophthora infestans Resistance gene 1 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Promoter
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BCH-GENE-SCBD-103778-2 Phytophthora infestans Resistance gene 1 terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
Expression cassettes:
The insertion contains three gene expression cassettes: Solanum bulbocastaneum Phytophthora infestans resistance gene 1 (Rpi-blb1), Solanum bulbocastaneum Phytophthora infestans resistance gene 2 (Rpi-blb2) and Arabidopsis thaliana acetohydroxyacid synthase (ahas).
Transcription of Rpi-blb1 and Rpi-blb2 are under control of the native promoters and terminators. Transcription of ahas is under control of the Agrobacterium tumefaciens nopaline synthase promoter and terminator. The R gene cassettes were originally excised from the donor genome as genomic fragments and inserted into the vector.
The coding sequence of ahas contains a point mutation S653N (serine to asparagine at amino acid position 653), which confers herbicide tolerance.
EN
The insertion contains three gene expression cassettes: Solanum bulbocastaneum Phytophthora infestans resistance gene 1 (Rpi-blb1), Solanum bulbocastaneum Phytophthora infestans resistance gene 2 (Rpi-blb2) and Arabidopsis thaliana acetohydroxyacid synthase (ahas).
Transcription of Rpi-blb1 and Rpi-blb2 are under control of the native promoters and terminators. Transcription of ahas is under control of the Agrobacterium tumefaciens nopaline synthase promoter and terminator. The R gene cassettes were originally excised from the donor genome as genomic fragments and inserted into the vector.
The coding sequence of ahas contains a point mutation S653N (serine to asparagine at amino acid position 653), which confers herbicide tolerance.
EN
- Food
Low level of expression of both Rpi-blb1 and Rpi-blb2 was detected by real-time PCR analysis in leaves, stems, tubers and roots. In flowers, low expression of Rpi-blb2 was detected. However, the expression of Rpi-blb1 was not detected in floral tissues.
Due to the nos promoter, low expression levels in all parts of the plant are expected for ahas. Expression of ahas was essential for the selection of transformants during tissue culturing.
EN
Due to the nos promoter, low expression levels in all parts of the plant are expected for ahas. Expression of ahas was essential for the selection of transformants during tissue culturing.
Vector information
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant Mol. Biol., 25, 989-994.
EN
Derivate of pPZP200. Reference: Hajdukiewicz et al (1994) Plant Mol. Biol., 25, 989-994.
- 07-r42-01-app-a1.pdf [ English ]
- European Patent Application - EP2535416A1.pdf [ English ]
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