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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Drought-tolerant sugarcane
EN
NXI-6T
No
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Surabaya,Organization:PT Perkebunan Nusantara XI (Persero)
60175, IndonesiaPhone: +62 031 3524596,Fax:Email: sekretariat@ptpn11.co.id,Website: https://ptpn11.co.id/,
The sugarcane (Saccharum officinarum L.) was modified for abiotic (drought, salt) stress tolerance through the expression of Rhizobium meliloti choline dehydrogenase, which leads to increased biosynthesis of the osmoprotectant glycine betaine that maintains the cell's water potential. The expression of choline dehydrogenase may also increase sugar content and promote early maturation. The sugarcane also contains Escherichia coli neomycin phosphotransferase II and hygromycin B phosphotransferase cassettes for kanamycin and hygromycin selection, respectively.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-115592-1 Organism Saccharum officinarum L. (Sugarcane, Sugar cane)Crops
Sugarcane cultivar JT26
EN
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Drought tolerant sugarcane| Changes in physiology and/or production (Ripening, Yield), Resistance to antibiotics (Hygromycin, Kanamycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought, Salinity)
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Drought tolerant sugarcane| Changes in physiology and/or production (Ripening, Yield), Resistance to antibiotics (Hygromycin, Kanamycin), Selectable marker genes and reporter genes, Tolerance to abiotic stress (Drought, Salinity)
pMLH2113
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-116036-1 Glutamate dehydrogenase mitochondrial transit peptide | Solanum lycopersicum (Tomato, SOLLC)Transit signal
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BCH-GENE-SCBD-116039-1 Choline dehydrogenase | Sinorhizobium meliloti (RHIML)Protein coding sequence | Changes in physiology and/or production (Ripening, Yield),Tolerance to abiotic stress (Drought, Salinity)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
The modified sugarcane contains three gene cassettes: Rhizobium meliloti choline dehydrogenase (betA); Escherichia coli neomycin phosphotransferase (nptII) and E. coli hygromycin B phosphotransferae (hph).
The betA sequence is under control of a Cauiflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. At the 5' end of the betA coding sequence is a Tobacco mosaic virus omega 5' leader to enhance translation and a Solanum lycopersicum glutamate dehydrogenase mitochondrial transit peptide, which directs the translated protein to the mitochondria.
The hph coding sequence is under transcriptional control of a CaMV 35S promoter and terminator.
The nptII coding sequence is under transcriptional control of a nos promoter and terminator.
Notes:
- The CaMV promoter associated with the betA coding sequence may have two tandem repeats of -419 to -90.
The betA sequence is under control of a Cauiflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (nos) terminator. At the 5' end of the betA coding sequence is a Tobacco mosaic virus omega 5' leader to enhance translation and a Solanum lycopersicum glutamate dehydrogenase mitochondrial transit peptide, which directs the translated protein to the mitochondria.
The hph coding sequence is under transcriptional control of a CaMV 35S promoter and terminator.
The nptII coding sequence is under transcriptional control of a nos promoter and terminator.
Notes:
- The CaMV promoter associated with the betA coding sequence may have two tandem repeats of -419 to -90.
EN
- Biofuel
- Food
EN
The events NXI-1T and NXT-4T were developed while producing NXT-6T.
- EUginius - NXI-6T sugarcane [ English ]
- ISAAA - NXI-6T sugarcane [ English ]
- Application No AU 200059458 B2 - Early-maturing sugarcane with high sugar content.pdf [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Related LMO(s) | 1 | |
