DP-3Ø5423-1 x MON-Ø4Ø32-6 - Modified fatty acid, herbicide-tolerant soybean | BCH-LMO-SCBD-101262 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 12 Jul 2019
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Modified fatty acid, herbicide-tolerant soybean
305423 x GTS 40-3-2
DP-3Ø5423-1 × MON-Ø4Ø32-6
  • - Person: Pioneer Hi-Bred | BCH-CON-MX-101254-2
    Pioneer Hi-Bred
    , United States of America
    Phone: (515) 270-3200,
    Email: @pioneer.com,
    Related Organization
    Pioneer Hi-Bred ()
    , United States of America
    Phone: (515) 270-3200,
    Email: @pioneer.com,
The stacked soy line DP-3Ø5423-1 x MON-Ø4Ø32-6 was obtained through the traditional cross breading of each of the parental organisms to produce a soy that expresses each of EPSPS, ALS and omega-6-fatty acid dehydrogenase genes. The expression of these genes are expected to confer tolerance to sulphonylurea and glyphosate herbicides and  increased levels of monounsaturated fatty acid and decreased levels of polyunsaturated fatty acids.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-ORGA-SCBD-10453-6 Organism Glycine max (Soybean, Soya bean, Soya, SOYBN)
  • BCH-LMO-SCBD-49073-9 Living Modified Organism DP-3Ø5423-1 - TREUS™Plenish™ Soybean
    Pioneer Hi-Bred Production Inc. | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to herbicides (Sulfonylurea)
  • BCH-LMO-SCBD-14796-14 Living Modified Organism MON-Ø4Ø32-6 - Roundup Ready™ soybean
    Monsanto | Resistance to herbicides (Glyphosate)
Characteristics of the modification process
PV-GMGT04, PHP19340 and PHP17752
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from GTS 40-3-2 vector PV-GMGT04
The soybean line GTS 40-3-2 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered soybean line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS 40-3-2 to survive the otherwise lethal application of glyphosate.

DNA insert from 305423 vector PHP19340 and PHP17752
The intended effect of the modification in 305423 soybean is to produce soybean seeds with increased levels of monounsaturated fatty acid and decreased levels of polyunsaturated fatty acids through the insertion of the microsomal omega-6 desaturase gene (FAD2-1) into soybean. Transcription of the gene fragment acts to silence the expression of the endogenous soybean omega-6 desaturase, which results in an increased level of oleic acid and decreased levels of linoleic and linolenic acids in the soybean seed. The acetolactate synthase (als gene) that confers tolerance to a sulfonylurea herbicide was used as a selectable marker for the transformation.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
  • BCH-GENE-SCBD-115046-3 omega-6-desaturase | (Soybeans)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
The DNA insert from  PHP19340 and PHP17752 contains a fragment corresponding to 40% of the soy omega-6-desaturase. Upon transcription of the fragment, the resulting transcript acts to silencing the endogenous gene by RNA interference.
  • Food
  • Feed
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 8
Laboratory for detection and identification of LMOs LMO(s) detectable by the laboratory 5
Risk Assessment generated by a regulatory process Living modified organism(s) 14
Living Modified Organism Related LMO(s) 2