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Living Modified Organism (LMO)
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BCH-LMO-SCBD-101526-7   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 20 Jan 2011 last updated: 19 Apr 2024
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Brassica napus modified for reduced sinapine content (pLH-BnSGT-GUS)
EN
pLH-BnSGT-GUS (line 1501.24)
No
In the GM oilseed rape plants that were transformed using the construct pLH-BnSGT-GUS the activity of the native oilseed rape enzyme UDP-glucose:sinapate glucosyltransferase (SGT) was reduced with the help of an RNAi construct. As a step in the biosynthesis of sinapine the UDP-glucose:sinapate glucosyltransferase catalyses the conversion of sinapic acid to sinapoyl glucose.

Suppression of the UDP-glucose:sinapate glucosyltransferase leads to a reduction of sinapine content in the seeds of the GM oilseed rape plants.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar/breeding line: Drakkar, Lisora
EN
Characteristics of the modification process
pLH-BnSGT-GUS Agrobacterium Ti plasmid derived
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-101523-2 UDP-glucose:sinapate glucosyltransferase | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-101521-6 Napin gene promoter | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
RNAi construct: 212 bp fragments of SGT-gen in sense and antisense orientation, seperated by a spacer (nt790-nt1812 of uidA)
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Related LMO(s) 1
Country's Decision or any other Communication LMO identification 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1