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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Brassica napus modified for reduced sinapine content (pLH7000-SGT/SCT)
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pLH7000-SGT/SCT (line 1505.1d)
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Organization:FINAB e. V. (outdated) ()OtherThünenplatz 1Groß Lüsewitz, Mecklenburg-Vorpommern
18190, GermanyPhone:Fax:Email: webmaster@finab.de,Website: http://www.finab.de,
In the GM oilseed rape plants that were transformed using the construct pLH7000-SGT/SCT, the activity of the native oilseed rape enzymes UDP-glucose:sinapate glucosyltransferase (SGT) and sinapoylglucose:choline sinapoyltransferase (SCT) was reduced with the help of an RNAi construct. In oilseed rape plants UDP-glucose:sinapate glucosyltransferase catalyses the conversion of sinapic acid to sinapoylglucose. Sinapoylglucose:choline sinapoyl-transferase (SCT) catalyses the conversion of sinapoylglucose to sinapoyl-choline (sinapine).
By suppressing UDP-glucose:sinapate glucosyltransferase and sinapoylglucose:choline si-napoyltransferase the sinapine content in the seeds of the GM oilseed rape plants is further reduced.
EN
By suppressing UDP-glucose:sinapate glucosyltransferase and sinapoylglucose:choline si-napoyltransferase the sinapine content in the seeds of the GM oilseed rape plants is further reduced.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12083-8 Organism Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Crops
Cultivar/breeding line: Drakkar, Lisora
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pLH7000-SGT/SCT
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-101519-2 Sinapoylglucose:choline sinapoyltransferase | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
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BCH-GENE-SCBD-101523-2 UDP-glucose:sinapate glucosyltransferase | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101521-6 Napin gene promoter | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
RNAi construct:
- seed specific short napin 590-promoter
- 197 bp fragment of the cDNA of the SCT-gen and 183 bp of the cDNA of the SGT-gen in sense orientation,
- nt790-nt1812 of uidA as a spacer, and 183 bp of the cDNA of the SGT-gen and 197 bp of the cDNA of SCT-gen in antisense orientation
- terminator of the nos gene.
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- seed specific short napin 590-promoter
- 197 bp fragment of the cDNA of the SCT-gen and 183 bp of the cDNA of the SGT-gen in sense orientation,
- nt790-nt1812 of uidA as a spacer, and 183 bp of the cDNA of the SGT-gen and 197 bp of the cDNA of SCT-gen in antisense orientation
- terminator of the nos gene.
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- Research
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| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
