Tobacco transformed with fission yeast mitotic activator | BCH-LMO-SCBD-102014 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 29 Jun 2011 last updated: 13 Aug 2012
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Tobacco transformed with fission yeast mitotic activator
EN
Transformation event unknown
No
Plants of Nicotiana tabacum were transformed with cdc25 gene from Schizosaccharomyces pombe under constitutive promotor 35S CaMV, together with an nptII gene for resistance to kanamycin. The transformation with the gene cdc25 significantly speeds up plant development and flowering earliness.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Nicotiana tabacum L. cv. Samsun
EN
Characteristics of the modification process
pBIN19
EN
  • Agrobacterium-mediated DNA transfer
 
0.800 kb
 
 
1.740 kb
 
 
0.200 kb
 
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-102013-3 Cdc25 gene | Schizosaccharomyces pombe (Fission yeast)
    Protein coding sequence | Changes in physiology and/or production
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
EN
LMO characteristics
EN
  • Research
Detection method(s)
- Polymerase chain reaction (PCR) with primers specific to inserted sequences
- Southern hybridization
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 1