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Living Modified Organism
(LMO)
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Tobacco transformed with fission yeast mitotic activator
EN
Transformation event unknown
No
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Person:Dennis Francis
CF10 3XQ, United Kingdom of Great Britain and Northern IrelandPhone: +44 29208 76145,Fax:Email: FrancisD@cardiff.ac.uk,Website:Related OrganizationCardiff University ()Academic or research instituteCardiff, Wales
CF10 3XQ, United Kingdom of Great Britain and Northern IrelandPhone: +44 29208 76145,Fax:Email: FrancisD@cardiff.ac.uk,Website:
Plants of Nicotiana tabacum were transformed with cdc25 gene from Schizosaccharomyces pombe under constitutive promotor 35S CaMV, together with an nptII gene for resistance to kanamycin. The transformation with the gene cdc25 significantly speeds up plant development and flowering earliness.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12120-4 Organism Nicotiana tabacum (Tobacco, TOBAC )Crops
Nicotiana tabacum L. cv. Samsun
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pBIN19
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-102013-3 Cdc25 gene | Schizosaccharomyces pombe (Fission yeast)Protein coding sequence | Changes in physiology and/or production
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
EN
EN
- Research
- Polymerase chain reaction (PCR) with primers specific to inserted sequences
- Southern hybridization
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- Southern hybridization
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