MON-8746Ø-4 - Droughtgard™ Maize | BCH-LMO-SCBD-103066 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 02 Jul 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Droughtgard™ Maize
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MON 87460
Yes
MON-8746Ø-4
  • - Organization: Monsanto Canada Inc. () | BCH-CON-CA-9841-2
    Organization:
    Monsanto Canada Inc. ()
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MON 87460 was developed through Agrobacterium-mediated transformation of conventional maize variety embryos and expresses cold shock protein B (CspB) from Bacillus subtilis and NptII from Tn5 of Escherichia coli. MON 87460 was developed to provide reduced yield loss under water-limited conditions compared to conventional maize.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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Characteristics of the modification process
PV-ZMAP595
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
A disarmed Agrobacterium tumefaciens plant transformation system including the double-border, binary vector PV-ZMAP595 was used to transform LH59 embryos producing MON 87460.

The disarmed Agrobacterium tumefaciens transformation vector PV-ZMAP595 contains both left and right T-DNA border sequences facilitating the transformation. It was constructed using standard molecular biology techniques. The T-DNA region of this vector contains cspB and nptII expression cassettes and it is the portion that is integrated to maize genome during transformation event.

The genetic elements of PV-ZMAP595 intended for insertion into the maize genome comprised between the T-DNA borders are, from the right border region, promoter and leader from the rice actin gene (P-Ract1), a non-translated intron from the rice actin gene (I-Ract1), the cspB coding sequence (CS-cspB) and a polyadenylation sequence from the transcript 7 gene (T-tr7). These elements together constitute the cspB expression cassette which is followed by the nptII expression cassette. The latter is flanked by two loxP sites and constitutes of a transcriptional promoter (P-35S), the nptII coding sequence (CS-nptII), and a polyadenylation sequence from the nopaline synthase gene (T-nos).

MON 87460 contains one copy of the insert at a single insertion site hosting both cspB and nptII intact expression cassettes. No additional elements from the transformation vector PV-ZMAP595, linked or unlinked to the cspB and nptII expression cassettes, were detected in the genome of MON 87460. Additionally, backbone sequence from the plasmid PV-ZMAP595 was not detected.
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LMO characteristics
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  • Food
  • Feed
  • Biofuel
Additional Information
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