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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Common bean modified for resistance to Bean Golden Mosaic Virus
EN
Embrapa 5.1
Yes
EMB-PVØ51-1
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Person:Mr Francisco José Lima AragãoCientist, National Research Center of Biotechnology ResearchParque Estação Biológica - Final da W5 Norte - Caixa Postal 02372Brasília, Distrito Federal
70770-900,Phone: 55 + 61 3448-4710, 55 + 61 3448-4706,Fax: 55+613340-3658,Email: aragao@cenargen.embrapa.br,Website: http://lattes.cnpq.br/6121745125946782,Related OrganizationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Parque Estação Biológica - Final da W5 Norte - Caixa Postal 02372Brasília, Distrito Federal
70770-900,Phone: 55 + 61 3448-4710, 55 + 61 3448-4706,Fax: 55+613340-3658,Email: aragao@cenargen.embrapa.br,Website: http://lattes.cnpq.br/6121745125946782, -
Person:Mr Josias Correa FariasCientist, National Center for Research of Rice and BeansRodovia Goiânia - Nova Veneza, Km 12 - Zona Rural. Caixa Postal 179Santo Antonio de Goiás, Goiás
75375-000,Phone: 55 (62) 3532-2110,Fax: 55 (62) 3533-2100,Email: josias@cnpaf.embrapa.br,Website: http://lattes.cnpq.br/5616359873902028,Related OrganizationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Rodovia Goiânia - Nova Veneza, Km 12 - Zona Rural. Caixa Postal 179Santo Antonio de Goiás, Goiás
75375-000,Phone: 55 (62) 3532-2110,Fax: 55 (62) 3533-2100,Email: josias@cnpaf.embrapa.br,Website: http://lattes.cnpq.br/5616359873902028,
The common bean (Phaseolus vulgaris L.) was genetically modified to exhibit resistance to the Bean Golden Mosaic Virus (BGMV).
RNA interference (RNAi) technology was used to impart resistance to BGMV by generating a construct containing a fragment of the AC1 gene of BGMV, positioned in sense/ antisense orientation.
This results in the transcript forming a double stranded RNA hairpin that is recognized by cellular machinery and thus initiates the degradation process of the dsRNA fragments. This ultimately results in the silencing of the AC1 gene which is essential for viral genome replication and consequently imparts resistance against the BGMV virus in the plant.
EN
RNA interference (RNAi) technology was used to impart resistance to BGMV by generating a construct containing a fragment of the AC1 gene of BGMV, positioned in sense/ antisense orientation.
This results in the transcript forming a double stranded RNA hairpin that is recognized by cellular machinery and thus initiates the degradation process of the dsRNA fragments. This ultimately results in the silencing of the AC1 gene which is essential for viral genome replication and consequently imparts resistance against the BGMV virus in the plant.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-104363-6 Organism Phaseolus vulgaris (String bean, French bean, Kidney bean, Common Bean , PHAVU)Crops
EN
pBGMVRNAiAHAS derived from vector pBLUESCRIPT II SK (-)
EN
- Biolistic / Particle gun
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-104598-2 Rolling-circle replication initiation factor | Bean golden mosaic virus (BGMV)Protein coding sequence | Resistance to diseases and pests
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BCH-GENE-SCBD-48073-8 Acetohydroxy acid synthase gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Resistance to herbicides (Imidazolinone, Sulfonylurea)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103123-6 Pyruvate orthophosphate dikinase, Intron 3 | Flaveria trinervia (Clustered Yellowtops, speedyweed, flaveria, yellow twinstem)Intron
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103933-4 Acetohydroxy acid synthase gene terminator | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Terminator
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BCH-GENE-SCBD-103932-4 Acetohydroxy acid synthase gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
The pBGMVRNAiAHAS vector also contained the beta-lactamase gene of Escherichia coli, this was however shown to be non functional in the Embrapa 5.1 line.
Three copies of the AHAS gene integrated into the host genome however the expression of this gene occurs at a level that is considered too low to impart significant herbicide tolerance in the Embrapa 5.1 line.
EN
Three copies of the AHAS gene integrated into the host genome however the expression of this gene occurs at a level that is considered too low to impart significant herbicide tolerance in the Embrapa 5.1 line.
EN
- Food
- Feed
EN
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
