DAS-68416-4 - Enlist™ Soybeans | BCH-LMO-SCBD-104808 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 27 Mar 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Enlist™ Soybeans
EN
DAS-68416-4
Yes
DAS-68416-4
DAS-68416-4 soybean was modified with the insertion of a gene encoding aryloxyalkanoate dioxygenase and phosphinothricin N-acetyltransferase to confer tolerance to the herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate respectively.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pDAB4468
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-104795-4 RB7 matrix attachment region | Nicotiana tabacum (Tobacco, TOBAC )
    Enhancer
  • BCH-GENE-SCBD-104802-5 Polyubiquitin10 gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-104805-2 Aryloxyalkanoate dioxygenase gene | Delftia acidovorans (DELAC)
    Protein coding sequence | Resistance to herbicides
  • BCH-GENE-SCBD-104806-3 ORF23 3' Untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101900-6 CsVMV promoter | Cassava vein mosaic virus (Cassava vein mosaic virus, CVMV, CsVMV)
    Promoter
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-104807-2 ORF1 3' Untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
Aryloxyalkanoate dioxygenase gene sequence from Delftia acidovorans was plant-optimized. The native and plant-optimized DNA sequences of aad-12 are 79.7% identical.

Southern blot analysis indicated that a single, intact insert of the aad-12 and pat genes were stably integrated into the soybean genome. Southern analyses also indicated the absence of the plasmid backbone DNA in DAS-68416-4 soybean.
EN
LMO characteristics
EN
  • Food
  • Feed
Detection method(s)
EN
Additional Information
EN
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