SHD-29821-8 - Colour modified carnation | BCH-LMO-SCBD-105857 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 28 Aug 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Colour modified carnation
EN
29821
Yes
SHD-29821-8
Carnation variety 29821 flowers have a novel colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia, and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
    Promoter
  • BCH-GENE-SCBD-43793-4 Flavonoid 3', 5'-hydroxylase gene | Viola sp. (Pansy, VIOLA)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
    Terminator
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-105436-1 Anthocyanin-3',5'-methyltransferase gene | Torenia sp. (Torenia hybrid, Wishbone flowers, Blue Wings, TOREN)
    Protein coding sequence | Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin), Pigmentation / Coloration)
29821 was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing four genes that function together in the biosynthesis of the anthocyanin pigments related to delphinidin.

The transgenic lines were derived from the parent cultivar which is a pink coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene , a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), and an anthocyanin-3',5'-methyltransferase gene.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce  anthocyanins with a novel hue.
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
Additional Information
EN
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