SHD-29821-8 - Colour modified carnation | BCH-LMO-SCBD-105857 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 28 Aug 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Colour modified carnation
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29821
Yes
SHD-29821-8
Carnation variety 29821 flowers have a novel colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia, and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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Characteristics of the modification process
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  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )
    Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
  • BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)
    Promoter
  • BCH-GENE-SCBD-43793-4 Flavonoid 3', 5'-hydroxylase gene | Viola sp. (Pansy, VIOLA)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)
    Terminator
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-105436-1 Anthocyanin-3',5'-methyltransferase gene | Torenia sp. (Torenia hybrid, Wishbone flowers, Blue Wings, TOREN)
    Protein coding sequence | Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin), Pigmentation / Coloration)
29821 was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing four genes that function together in the biosynthesis of the anthocyanin pigments related to delphinidin.

The transgenic lines were derived from the parent cultivar which is a pink coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene , a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), and an anthocyanin-3',5'-methyltransferase gene.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce  anthocyanins with a novel hue.
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LMO characteristics
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  • Ornamental
Detection method(s)
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Additional Information
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