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Living Modified Organism
(LMO)
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Tobacco modified for xylanase production
EN
XynZ No 34 and XynZ No 46
No
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Organization:Institut für Pflanzengenetik und Kulturpflanzenforschung ()Corrensstrasse 3Gatersleben,
06466 , GermanyPhone: +49 (0)39482 5-0 ,Fax: +49 (0) 39482 5139,Email: info@ipk-gatersleben.de,Website: http://www.ipk-gatersleben.de,
The genetically modified tobacco plants constitutively express a shortened version of the xylanase gene from Clostridium thermocellum that is N-terminally fused to the signal peptide of the proteinase inhibitor II from potato, which leads to the gene product being into the apoplast.
Xylan is a component of the hemicellulose of plant cell walls. It is degraded by xylanases. The transport into the apoplast removes the xylanase from the protease-rich intracellular space, preventing it from being degraded.
Despite the presence of the xylanase, no alteration in plant growth, susceptibility to pathogens and cell wall composition was observed in the transgenic tobacco plants.
EN
Xylan is a component of the hemicellulose of plant cell walls. It is degraded by xylanases. The transport into the apoplast removes the xylanase from the protease-rich intracellular space, preventing it from being degraded.
Despite the presence of the xylanase, no alteration in plant growth, susceptibility to pathogens and cell wall composition was observed in the transgenic tobacco plants.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12120-4 Organism Nicotiana tabacum (Tobacco, TOBAC )Crops
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Derivative of pBIN19
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-110883-1 Proteinase inhibitor II transit peptide | Solanum tuberosum (Potato, SOLTU)Transit signal
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BCH-GENE-SCBD-111594-1 Xylanase Z gene | Ruminiclostridium thermocellum (CLOTM)Protein coding sequence | Use in industrial applications
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
EN
EN
- Research
EN
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