Tobacco modified for xylanase production | BCH-LMO-SCBD-111621 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 06 Feb 2017
Living Modified Organism identity
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Tobacco modified for xylanase production
XynZ No 34 and XynZ No 46
The genetically modified tobacco plants constitutively express a shortened version of the xylanase gene from Clostridium thermocellum that is N-terminally fused to the signal peptide of the proteinase inhibitor II from potato, which leads to the gene product being into the apoplast.

Xylan is a component of the hemicellulose of plant cell walls. It is degraded by xylanases. The transport into the apoplast removes the xylanase from the protease-rich intracellular space, preventing it from being degraded.

Despite the presence of the xylanase, no alteration in plant growth, susceptibility to pathogens and cell wall composition was observed in the transgenic tobacco plants.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
Derivative of pBIN19
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
LMO characteristics
  • Research
Detection method(s)
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