Petunia modified for altered flower colour | BCH-LMO-SCBD-112261 | Living Modified Organism | Biosafety Clearing-House

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BCH-LMO-SCBD-112261-1   |   PDF   |   Print   |  

Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 13 Jul 2017
Living Modified Organism identity
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Petunia modified for altered flower colour
EN
Unknown
No
Unauthorized petunias that have been modified for altered flower color were found and tested for the presence of introduced genetic elements.

The petunias tested positive for the presence of several genetic elements: CS-dfr-MAIZE, P-35S-CaMV, P-nos-RHIRD, T-35S-CaMV, CS-nptII-ECOLX and T-ocs-RHIRD.

They also tested positive for the junctions between the elements P-nos-RHIRD to CS-nptII-ECOLX and P-35S-CaMV to CS-dfr-MAIZE.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • Petunia with a modified flower colour
    | Max Planck Institute for Plant Breeding Research(MPIPZ) | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin)
Characteristics of the modification process
EN
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-111603-2 Dihydroflavonol-4-reductase | Zea mays (Maize, Corn, MAIZE)
    Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
In addition to CS-dfr-MAIZE the petunias have been tested positive for several of the listed genetic elements. The order of the genetic elements in these gm petunia varieties is not known. Moreover, it is possible that further genetic elements are present in one or more of the gm petunia varieties.

The insert of the petunia varieties Bonnie Orange/African Sunset were fully characterised by Bashandy et. al. In these varieties the elements are arranged in the following order: P-35S-CaMV; CS-dfr-MAIZE; T-35S-CaMV; P-nos-RHIRD; CS-nptII-ECOLX; T-ocs-RHIRD.
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
There are no event specific methods available. However, element specific and construct-specific methods are available in the EUginius database.
EN
Additional Information
The precise identity and origin of the LM petunia is unknown and, as such, no developer is listed for this LMO.

A list of all currently known genetically modified petunia varieties identified in Germany or in the Netherlands is provided below.

Bashandy et. al sequenced the petunia variety Bonnie Orange/African Sunset insert; the sequencing results are available at GenBank (KY964325.1).
EN
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