DP-3Ø5423-1 x MON-877Ø8-9 x MON-89788-1 - High oleic acid, herbicide tolerant soy | BCH-LMO-SCBD-114651 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 12 Jul 2019
Living Modified Organism identity
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High oleic acid, herbicide tolerant soy
DP-3Ø5423-1 × MON-877Ø8-9 × MON-89788-1
  • - Organization: Dupont Poineer () | BCH-CON-SCBD-106199-2
    Dupont Poineer ()
    Private sector (business and industry)
    Chestnut Run Plaza 720/1S5 974 Centre Road
    Wilmington,, Delaware
    19805, United States of America
The soybean has been modified to produce increased levels of monounsaturated fatty acid (oleic) and decreased levels of polyunsaturated fatty acids (linoleic and linolenic). The additional inserted genes (acetolactate synthase, dicamba monooxygenase, and cp4 epsps) confer tolerance to herbicides (sulfonylurea, 3,6-dichloro-2-methoxybenzoic acid, and glyphosate, respectively).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
PHP19340 and PHP17752; PV-GMHT4355;  PV-GMGOX20
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Genetic elements associated with PHP19340 and PHP17752:
Microprojectile bombardment was used to co-transform secondary plant cell embryos with two purified linear DNA fragments: a 2924 base pair fragment (PHP19340A fragment) containing the gm-fad2-1 cassette, and the 4512 base pair fragment (PHP17752A fragment) containing the gm-hra cassette. The gm-fad2-1 cassette includes the promoter region from the Glycine max (soybean) Kunitz trypsin inhibitor gene (KTi3), a fragment of the  omega(6)-fatty acid dehydrogenase (FAD2-1) gene that corresponds to approximately 40% of the middle portion of the FAD2-1, and the 3' untranslated region of the KTi3 gene (KTi3 terminator).The gm-hra cassette contains the gm-hra gene, which is an optimized form of the endogenous Acetohydroxy acid Synthase (als) gene from soybean, with transcription regulated by the S-adenosyl-L-methionine synthetase (SAMS) constitutive promoter from soybean and with transcription terminated by the endogenous als gene terminator from soybean.

Note: Sequence characterization of the inserted DNA in 305423 soybean confirms that it contains four insertions that comprise:
    * Insertion 1: one truncated PHP19340A fragment with a truncated KTi3 terminator and intact gm-fad2-1 gene fragment and intact KTi3 promoter, one intact PHP19340A fragment, one intact PHP17752A fragment, one truncated PHP19340A fragment with an intact KTi3 promoter and a truncated gm-fad2-1 gene fragment, and one truncated PHP19340A fragment with a truncated KTi3 promoter and truncated gm-fad2-1 gene fragment.
    * Insertion 2: one truncated PHP19340A fragment with a truncated KTi3 promoter and with intact gm-fad2-1 gene fragment and intact KTi3 terminator.
    * Insertion 3: one truncated copy of the KTi3 promoter with a nonfunctional 495 bp fragment of the plasmid backbone; and
    * Insertion 4: two truncated PHP19340A fragments in an inverted repeat configuration, both with a truncated KTi3 promoter and intact gmfad2-1 gene fragment and KTi3 terminator.

Genetic elements associated with PV-GMHT4355:
Expression of the  Stenotrophomonas maltophilia dicamba monooxygenase (DMO) gene is controlled by the peanut chlorotic streak virus Full-Length transcript promoter and the Pisum sativum (pea) small subunit of rubulose-1,5- bisphosphate carboxylase terminator. The transcript contains 5' untranslated region of the tobacco etch virus (to improve gene expression), pea N-terminal chloroplast transit peptide (for chloroplast localization), and DMO.

- Southern blot analysis indicated that the second 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) expression cassette was not integrated into the MON87708 line and neither were any elements of the vector backbone. The cassette contained the following elements in an antisense orientation to the dicamba expression cassette: P-FMV>> L-DnaK>> TS-CTP2>> CS-C4 EPSPS>> T-E9.
- Southern blot analysis and sequencing analysis indicated that a single intact dicamba expression cassette was integrated into the MON87708 line. Southern blot analysis indicated that the vector backbone was not present in the parental line.

Genetic information associated with PV-GMGOX20:
Information on the inserted DNA sequences:
The DNA inserted into the soybean genome contains:
- Codon optimized coding sequence of the aroA (epsps) gene from the Agrobacterium sp. strain CP4 encoding the CP4 EPSPS protein.
- a chimeric transcriptional promoter (P-FMV/Tsf1) consisting of chimeric sequence derived from Arabidopsis thaliana Elongation factor 1 alpha (Tsf1) gene promoter and enhancer sequences from the 35S of the Figwort Mosaic Virus.
Located between the promoter and the cp4epsps coding sequence are:
- the non-translated leader sequence (L-Tsf1) and the I-Tsf1 non translated intron;
- a chloroplast transit peptide sequence (TS-CTP2), derived from the A. thaliana epsps gene and placed before the cp4 epsps gene encoding sequence in the gene insert;
- a polyadenylation sequence from RbcS2 gene (T-E9), derived from Pisum sativum (pea) containing the 3' non translated region of the pea ribulose-1,5 biphosphate carboxylase small sub unit E9.

- Southern blot analyses indicated that the parental MON 89788 contained a single intact cp4 epsps expression cassette integrated at a single locus within the soybean genome. DNA sequencing analyses of the MON 89788 insert confirmed the expected coding region of the cp4 epsps gene cassette, is identical to that transformed in the T-DNA cassette. No backbone sequences from the transformation plasmid were detected and no partial genetic elements, linked or unlinked to the inserted expression cassette were detected.

The genetic material that is inserted in 305423 soybean is genetically linked and segregates following a typical pattern of Mendelian inheritance expected for a single, genetically-linked insertion locus.PV-GMGOX20
LMO characteristics
  • BCH-GENE-SCBD-115046-3 omega-6-desaturase | (Soybeans)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
Transcription of the gene fragment under the control of a seed-preferred KTi3 promoter acts to silence the expression of the endogenous soybean omega-6 desaturase, which results in an increased level of oleic acid and decreased levels of linoleic and linolenic acids in the soybean seed.
  • Food
  • Feed
  • Biofuel
Detection method(s)
Please refer to the parental organism records for additional information on detection and identification.
Additional Information
Please refer to the parental organism records for more additional information.
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 2
Living Modified Organism Related LMO(s) 2
Country's Decision or any other Communication Living modified organism(s) 1