DP-3Ø5423-1 x MON-89788-1 - Herbicide-tolerant soybean with high oleic acid content | BCH-LMO-SCBD-115047 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 15 Jul 2019
Living Modified Organism identity
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Herbicide-tolerant soybean with high oleic acid content
DP305423 x MON89788
DP-3Ø5423-1 × MON-89788-1
  • - Organization: Dupont Poineer () | BCH-CON-SCBD-106199-2
    Dupont Poineer ()
    Private sector (business and industry)
    Chestnut Run Plaza 720/1S5 974 Centre Road
    Wilmington,, Delaware
    19805, United States of America
The soybean is the result of a stacked event, created through the cross between TREUS™Plenish™ and Roundup Ready2Yield™ soybean lines. The modified soy contains Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase for tolerance to the herbicide glyphosate, Glycine max acetohydroxy acid synthase for sulfonylurea herbicide tolerance, and a silencing construct to reduce the expression of endogenous ω-6 desaturase to reduce the production of polyunsaturated fatty acids.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-LMO-SCBD-49073-9 Living Modified Organism DP-3Ø5423-1 - TREUS™Plenish™ Soybean
    Pioneer Hi-Bred Production Inc. | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to herbicides (Sulfonylurea)
  • BCH-LMO-SCBD-40284-18 Living Modified Organism MON-89788-1 - Roundup Ready2Yield™ soybean
    Monsanto | Resistance to herbicides (Glyphosate)
  • DP-3Ø5423-1 x MON-877Ø8-9 x MON-89788-1 - High oleic acid, herbicide tolerant soy
    | Dr. Wendelyn Jones | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to herbicides (Glyphosate, Sulfonylurea), Tolerance to dicamba (3,6-dichloro-2-methoxybenzoic acid) herbicide.
  • DP-3Ø5423-1 x MON-Ø4Ø32-6 - Modified fatty acid, herbicide-tolerant soybean
    | Pioneer Hi-Bred Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to herbicides - Glyphosate, Sulfonylurea
Characteristics of the modification process
PHP19340 and PHP17752; PV-GMGOX20
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Genetic elements associated with PHP19340 and PHP17752:
Two linear DNA fragments were introduced to the parental soybean (Glycine max) line using microparticle bombardment:
-PHP19340A, a 2924 base pair fragment containing Glycine max microsomal omega-6-desaturase (FAD2-1) cassette; 
-PHP17752A, a 4512 base pair fragment containing the gm-hra (modified soybean acetohydroxy acid synthase (als) gene) cassette.

The fad2-1 cassette is under transcriptional control by the soybean Kunitz trypsin inhibitor gene (KTi3) and the KTi3 terminator. The fad2-1 fragment present in the cassette corresponds to a 40% of the middle portion of the endogenous gene. The cassette does not code for a protein.

The gm-hra cassette contains the coding sequence of als under transcriptional control of the soybean S-adenosyl-L-methionine synthetase (SAMS) constitutive promoter the endogenous als terminator. The coding sequence of gm-hra was produced by optimizing als.

Four insertions were present in the parental line:
- Insertion 1: one truncated PHP19340A fragment with a truncated KTi3 terminator and intact fad2-1 fragment and intact KTi3 promoter, one intact PHP19340A fragment, one intact PHP17752A fragment, one truncated PHP19340A fragment with an intact KTi3 promoter and a truncated fad2-1 fragment, and one truncated PHP19340A fragment with a truncated KTi3 promoter and truncated fad2-1 fragment.
- Insertion 2: one truncated PHP19340A fragment with a truncated KTi3 promoter and with intact fad2-1 fragment and intact KTi3 terminator.
- Insertion 3: one truncated copy of the KTi3 promoter with a non-functional 495 bp fragment of the plasmid backbone; and
- Insertion 4: two truncated PHP19340A fragments in an inverted repeat configuration, both with a truncated KTi3 promoter and intact fad2-1 fragment and KTi3 terminator.

Genetic information associated with PV-GMGOX20:
The DNA inserted into the soybean genome contains:
- Codon optimized coding sequence of the aroA (epsps) gene from the Agrobacterium tumefaciens strain CP4 encoding the CP4 EPSPS protein.
- a chimeric transcriptional promoter (P-FMV/Tsf1) consisting of chimeric sequence derived from Arabidopsis thaliana Elongation factor 1 alpha (Tsf1) gene promoter and enhancer sequences from the 35S of the Figwort Mosaic Virus.

Located between the promoter and the cp4epsps coding sequence are:
- the non-translated leader sequence (L-Tsf1) and the I-Tsf1 non translated intron;
- a chloroplast transit peptide sequence (TS-CTP2), derived from the A. thaliana epsps and placed before the cp4 epsps encoding sequence in the gene insert;

Transcription terminates at the polyadenylation sequence from RbcS2 (T-E9), derived from Pisum sativum (pea) containing the 3' non-translated region of the pea ribulose-1,5 biphosphate carboxylase small subunit E9.

- Southern blot analyses indicated that the parental MON89788 contained a single intact cp4 epsps expression cassette integrated at a single locus within the soybean genome. DNA sequencing analyses of the MON 89788 insert confirmed the expected coding region of the cp4 epsps gene cassette, is identical to that transformed in the T-DNA cassette. No backbone sequences from the transformation plasmid were detected and no partial genetic elements, linked or unlinked to the inserted expression cassette were detected.

For more information, please refer to the records of the parental lines.
LMO characteristics
  • BCH-GENE-SCBD-115046-3 omega-6-desaturase | (Soybeans)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
Under the control of a seed-preferred KTi3 promoter, transcription of the omega-6 desaturase fragment acts to silence the expression of the endogenous soybean omega-6 desaturase via RNA interference. The reduction in expression of the endogenous gene impedes biosynthesis of polyunsaturated fatty acids. Thus, the fatty acid composition in the soybean seeds is changed (increased proportion of oleic acid and decreased proportions of linoleic and linolenic acids).
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