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Living Modified Organism (LMO)
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BCH-LMO-SCBD-115067-3   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 26 Jul 2019 last updated: 18 Nov 2025
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Glyphosate and Glufosinate tolerant soybean
EN
DBN9004
Yes
DBN-Ø9ØØ4-6
The soybean (Glycine max) was modified for glyphosate and glufosinate herbicide tolerance. To achieve tolerance to glyphosate, the soybean expresses 5-enolpyruvylshikimate-3-phosphate synthase from the Agrobacterium tumefaciens CP4 strain, which has a reduced binding affinity for the herbicide and allows the continued functioning of the shikimate pathway. To achieve tolerance to glufosinate, the soybean expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pDBN4003
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-115065-1 Elongation Factor 1 alpha promoter | Glycine max (Soybean, Soya bean, Soya, SOYBN)
    Promoter
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
Information of the inserted DNA sequences
The transforming plasmid pDBN4003 carried a transfer DNA  comprising of two gene cassettes expressing Agrobacterium tumefaciens CP4 strain 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).

(1) Transcription of the cp4 epsps coding sequence is directed by the elongation factor 1 alpha polypeptide promoter from Glycine max, an Arabidopsis thaliana chloroplast transit peptide 2 of the ShkG gene sequence, and the Pisum sativum ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) E9 gene terminator. Elevated levels of transcription of cp4 epsps are expected due to the elongation factor 1 promoter. The EPSPS protein is expected to accumulate in the chloroplast due to the transit signal peptide.

(2) The transcription of pat coding sequence is under the control of the Cauliflower mosaic virus 35S promoter and the 35S terminator. Elevated levels of transcription of pat are expected due to the Cauliflower mosaic virus 35S promoter. The protein is expected to remain in the cytoplasm of the plant cells.


Notes
  • Southern analysis showed a single copy of the T-DNA insert (4799 bp) at a single genomic locus on chromosome 13 containing the two expression cassettes and the stable integration across generations, and confirmed the absence of plasmid sequences;
  • Sequencing analyses identified an intergenic 1545 bp deletion occurred at the insertion site, and bioinformatic and segregation analyses indicated no disruptions to any known endogenous soybean genes. that DBN9004 contains a single nuclear insertion that does not disrupt any known endogenous soybean genes.
EN
LMO characteristics
EN
  • Biofuel
  • Feed
  • Food
Detection method(s)
EN
Additional Information
Note that the European Food Safety Authority and the Netherlands Commission on Genetic Modification state Beijing DaBeiNong Biotechnology Co., Ltd. (DBNBC) as the applicant. Euginius and OECD Biotrack database list both Instituto de Agrobiotecnología Rosario (INDEAR) (Biosidus and Bioceres) and Beijing DaBeiNong Biotechnology Co., Ltd. (DBNBC) as the applicants.
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication LMO identification 2
Risk Assessment generated by a regulatory process Living modified organism(s) 2

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