DBN-Ø9ØØ4-6 - Glyphosate and Glufosinate tolerant soybean | BCH-LMO-SCBD-115067 | Living Modified Organism | Biosafety Clearing-House

Living Modified Organism (LMO)
Decisions on the LMO Risk Assessments  
published: 26 Jul 2019 last updated: 09 May 2023
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Glyphosate and Glufosinate tolerant soybean
The soybean (Glycine max) was modified for herbicide tolerance. For tolerance to glyphosate, the soybean expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which has a reduced binding affinity for the herbicide and allows the continued functioning of the shikimate pathway. For tolerance to glufosinate (L-phosphinothricin), the soybean expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase gene, which detoxifies the herbicide through acetylation  of the primary amino group.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-115065-1 Elongation Factor 1 alpha promoter | Glycine max (Soybean, Soya bean, Soya, SOYBN)
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
The modified soybean contains two gene cassettes: Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).

Transcription of the epsps commences from a Glycine max elongation factor 1 alpha promoter and terminates at a Pisum sativum ribulose-1,5-bisphosphate carboxylase small subunit terminator. An Arabidopsis thaliana chloroplast transit peptide 2 sequence was added to the 5' end of epsps coding sequence to target the translated protein to the chloroplast. Due to the nature of the promoter, high levels of transcription are expected.

Transcription of the pat coding sequence under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a CaMV terminator. Elevated levels of transcription of pat are expected due to the CaMV 35S promoter. The protein is expected to remain in the cytoplasm of the plant cells.
LMO characteristics
  • Biofuel
  • Feed
  • Food
Detection method(s)
Additional Information
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1