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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Papaya modified for resistance to Papaya Dieback Disease
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Person:Dr. Rogayah SekeliPlant Transformation and Molecular Biologist, Biotechnology and Nanotechnology Research CentreMARDI Headquarters, Persiaran MARDI-UPMSerdang, Selangor
43400, MalaysiaPhone: +603 89537313,Fax:Email: lynn@mardi.gov.my,Website:Related OrganizationMalaysian Agricultural Research and Development Institute (MARDI)Academic or research instituteMARDI Headquarters, Persiaran MARDI-UPMSerdang, Selangor
43400, MalaysiaPhone: +603 89537313,Fax:Email: lynn@mardi.gov.my,Website:
The Eksotika papaya (Carica papaya L. var. Eksotika) has been modified to have anti-pathogenic characteristics against Erwinia mallotivora, the causal agent of Papaya Dieback Diease, through the insertion of the two acyl-homoserine lactonase genes from Bacillus cereus CHB37 and Bacillus thuringiensis SP24. The two acyl-homoserine lactonase genes disrupt quorum sensing (bacterial cell-to-cell communication) through the hydrolysis of acyl-homoserine lactone and thus reducing expression of virulence genes and disease symptoms. Acyl-homoserine lactonase was previously found to specifically inhibit the growth of E. mallotivora in vitro, as well as in planta through the expression of B. cereus acyl-homoserine lactonase.
Additionally, Escherichia coli neomycin phophotransferase II, which confers resistance to kanamycin, was also inserted into the papaya genome to facilitate screening and identification of the transformants during the growth of the transgenic plants.
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Additionally, Escherichia coli neomycin phophotransferase II, which confers resistance to kanamycin, was also inserted into the papaya genome to facilitate screening and identification of the transformants during the growth of the transgenic plants.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12085-4 Organism Carica papaya (Papaya, Pawpaw, Papaw, CARPA)Crops
Variety Eksotika
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pCAMBIA2301
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- Agrobacterium-mediated DNA transfer
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0.346 kb
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0.753 kb
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0.253 kb
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0.180 kb
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0.800 kb
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0.680 kb
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0.346 kb
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0.753 kb
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0.253 kb
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0.175 kb
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0.798 kb
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0.678 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-101504-4 CaMV 35S promoter plus four repeats of activating sequence | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-115338-4 Acyl-homoserine lactonase CHB37 | Bacillus cereus CHB37Protein coding sequence | Resistance to diseases and pests (Bacteria),Resistance to Gram-negative bacteria
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BCH-GENE-SCBD-115339-2 Acyl-homoserine lactonase SP24 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Bacteria),Resistance to Gram-negative bacteria
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
Expression of acyl-homoserine lactonase genes:
Transcription of the Bacillus cereus CHB37 Acyl-homoserine lactonase is under the control of the Cauliflower Mosaic Virus 35S (CaMV 35S) promoter and the Agrobacterium tumefaciens nopaline synthase terminator (T-nos). Similarly, transcription of the Bacillus thuringiensis SP24 Acyl-homoserine lactonase is also under the control of the CaMV 35S promoter and the T-nos. High levels of expression are expected from the constitutive CaMV 35S promoter.
Selectable marker:
The vector contains an Escherichia coli neomycin phosphotransferase II cassette under transcriptional control of a CaMV 35S enhanced promoter and a CaMV 35S terminator. The CaMV 35S enhanced promoter is expected to promote constitutive expression of the neomycin phosphotransferase II transcript.
Please note:
The introduction of the two acyl-homoserine lactonase genes were a result of two separate Agrobacterium-mediated transformation events with the same vector.
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Transcription of the Bacillus cereus CHB37 Acyl-homoserine lactonase is under the control of the Cauliflower Mosaic Virus 35S (CaMV 35S) promoter and the Agrobacterium tumefaciens nopaline synthase terminator (T-nos). Similarly, transcription of the Bacillus thuringiensis SP24 Acyl-homoserine lactonase is also under the control of the CaMV 35S promoter and the T-nos. High levels of expression are expected from the constitutive CaMV 35S promoter.
Selectable marker:
The vector contains an Escherichia coli neomycin phosphotransferase II cassette under transcriptional control of a CaMV 35S enhanced promoter and a CaMV 35S terminator. The CaMV 35S enhanced promoter is expected to promote constitutive expression of the neomycin phosphotransferase II transcript.
Please note:
The introduction of the two acyl-homoserine lactonase genes were a result of two separate Agrobacterium-mediated transformation events with the same vector.
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- Food
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- Acyl homoserine lactonase genes from Bacillus species isolated from tomato rhizosphere soil in Malaysia.pdf [ English ]
- Quorum Quenching Bacteria Isolated from Rice and Tomato Rhizosphere Soil in Malaysia.pdf [ English ]
- Enhancing Eksotika Papaya Resistance to Dieback Disease through Quorum Quenching.pdf [ English ]
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