ACS-BNØØ8-2 - Herbicide Tolerant Canola | BCH-LMO-SCBD-14762 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 05 Jun 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide Tolerant Canola
EN
T45 (HCN28)
ACS-BNØØ8-2
Canola  modified for glufosinate tolerance with the insertion of the phosphinothricin acetyltransferase (pat) gene from Streptomyces viridochromogenes, conferring tolerance to phosphinothricin (Glufosinate ammonium) herbicide.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pHoe4/Ac
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The pat gene is a synthetic version of the gene isolated from Streptomyces viridochromogenes, strain Tü 494. The nucleotide sequence has been modified to provide codons preferred by plants without changing the amino acid sequence of the enzyme.

Southern blot analysis indicated that a single copy of the transformation cassette was integrated into the host genome with no integration of any portions of the vector backbone.
EN
LMO characteristics
EN
  • Food
  • Feed
Additional Information
The canola line T45 was genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.

Glufosinate tolerance in T45 is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. The PAT enzyme is not known to have any toxic properties. Line HCN28 was derived from T45.
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