CGN-89322-3 - Delayed-ripening tomato | BCH-LMO-SCBD-14781 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 17 Jan 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Delayed-ripening tomato
Tomato line 8338 was developed by introducing into the genome of a processing tomato cultivar UC82B a gene for 1-amino-cyclopropane-1-carboxylic acid deaminase (ACCd) derived from a nonpathogenic soil bacterium (Pseudomonas chlororaphis). In the plant, this enzyme catalyzes metabolism of 1-amino-cyclopropane-1-arboxylic acid (ACC), an essential precursor for the biosynthesis of the plant ripening hormone ethylene.

The initiation and progression of tomato fruit ripening depends on increased levels of ethylene. In line 8338, ACC is sufficiently reduced in detached fruit so that ethylene becomes limiting and the ripening process is delayed. Line 8338 has also been transformed with the nptII gene derived from Escherichia coli that encodes the enzyme NPTII. NPTII confers resistance to certain antibiotics, such as kanamycin, that are used to select transformed cells.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar: UC82B
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
1-amino-cyclopropane-1-carboxylic acid deaminase - Pseudomonas chlororaphis strain 6G5
LMO characteristics
  • Food
Detection method(s)
Additional Information