ACS-GMØØ6-4 - Liberty Link™ soybean | BCH-LMO-SCBD-14857 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 21 Jan 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Liberty Link™ soybean
EN
A5547-127
ACS-GMØØ6-4
The soybean line A5547-127 was developed to allow for the use of glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Ignite®, Rely®, Liberty®, Harvest®, and Finale®) as a weed control option. This genetically engineered soybean line contains the fungal enzyme phosphinothricin-N-acetyltransferase (PAT), which allows these plants to survive the otherwise lethal application of glufosinate. The pat gene inserted into A5547-127 was isolated from a common soil fungus, Streptomyces viridochromogenes, and introduced into the soybean genome by particle acceleration (biolistic) transformation.

The PAT enzyme in soybean line A5547-127 converts L-phosphinothricin (PPT), the active ingredient in glufosinate ammonium, to an inactive form thereby conferring resistance to the herbicide. In the absence of PAT, application of glufosinate leads to reduced production of the amino acid glutamine and increased ammonia levels in the plant tissues, which are lethal to the plant. The PAT enzyme is not known to have any toxic properties.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • ACS-GMØØ5-3 - Herbicide-tolerant soybean
    | Resistance to herbicides - Glufosinate
  • ACS-GMØØ1-8 - Herbicide-tolerant soybean
    | Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
  • ACS-GMØØ2-9 - Herbicide-tolerant soybean
    | Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
  • ACS-GMØØ3-1 - Herbicide-tolerant soybean
    | Resistance to herbicides - Glufosinate
  • ACS-GMØØ4-2 - Herbicide-tolerant soybean
    | Resistance to herbicides - Glufosinate
  • ACS-GMØØ8-6 - Herbicide-tolerant soybean
    | Resistance to herbicides - Glufosinate
Characteristics of the modification process
pB2/35SAcK (AKA: pWRG5143)
EN
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The PAT gene from Streptomyces viridochromogenes was synthetically modified with plant preferred codons.

The bacterial ampicillin resistance gene was also partially integrated into the host genome. It is however not expressed in the host organism.
EN
LMO characteristics
EN
  • Food
Detection method(s)
Southern blot analysis indicated that 1 copy of the PAT gene integrated into the host genome.

One copy of the 5' portion of the bla gene was integrated upstream of the PAT gene and one copy of the 3' portion of the bla gene was integrated downstream of the PAT gene.
EN
Additional Information
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Record type Field Record(s)