The recipient organism was co-transformed with the pRVA9909 and pDE110 vectors. The pRVA9909 vector contained the transformation cassette for Cry9C and the pDE110 vector contained the transformation cassette for the bar gene.

Southern blot analysis indicated that the LMO contains at least one intact copy of the Cry9c expression cassette and at least 4 copies of the PAT expression cassette one of which integrated into the genome without its regulatory elements.
The test also confirmed that there was integration of at least 4 copies of the bacterial β-lactamse gene. However this is non-functional in plant tissues.

The Cry9c DNA sequence was modified to truncate the C-terminal amino acids following amino acid position 666 and the first 43 amino acids at the N-terminal. Additionally the argenine amino acid at position 123 was modified to a lysine. These modifications do not affect the insecticidal activity of the protein.