ACS-ZMØØ4-3 - Starlink™ maize | BCH-LMO-SCBD-14862 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 18 Apr 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Starlink™ maize
EN
CBH-351
Yes
ACS-ZMØØ4-3
The transgenic Starlink™ maize line CBH-351 was developed to for insect resistance and glufosinate herbicide tolerance. Resistance to lepidopteran pests was confereed through the insertion of the Cry9C coding sequence. Tolerance to glufosinate is conferred through the insertion of the coding sequence for phosphinothricin N-acetyltransferase (PAT).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pRVA9909 and pDE110
EN
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14996-8 Cry9C | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis))
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-101901-3 5' untranslated leader of chlorophyll a/b-binding protein | Petunia hybrida (Petunia, PETHY)
    Leader
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
The recipient organism was co-transformed with the pRVA9909 and pDE110 vectors. The pRVA9909 vector contained the transformation cassette for Cry9C and the pDE110 vector contained the transformation cassette for the bar gene.

Southern blot analysis indicated that the LMO contains at least one intact copy of the Cry9c expression cassette and at least 4 copies of the PAT expression cassette one of which integrated into the genome without its regulatory elements.
The test also confirmed that there was integration of at least 4 copies of the bacterial β-lactamse gene. However this is non-functional in plant tissues.

The Cry9c DNA sequence was modified to truncate the C-terminal amino acids following amino acid position 666 and the first 43 amino acids at the N-terminal. Additionally the argenine amino acid at position 123 was modified to a lysine. These modifications do not affect the insecticidal activity of the protein.
EN
LMO characteristics
EN
  • Feed
Detection method(s)
EN
Additional Information
EN
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