Chicory RM3-3 modified for tolerance to herbicides | BCH-LMO-SCBD-15402 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 09 Apr 2014
Living Modified Organism identity
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Chicory RM3-3 modified for tolerance to herbicides
EN
RM3-3
No
Chicory male-sterility system displaying glufosinate herbicide tolerance. Contains the barnase gene from Bacillus amyloliquefaciens and the bar gene encoding phosphinothricin N-acetyltransferase from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
  • Chicory RM3-4 modified for tolerance to herbicides
    | Changes in physiology and/or production (Reproduction, Male sterility), Resistance to herbicides (Glufosinate)
  • Chicory RM3-6 modified for tolerance to herbicides
    | Changes in physiology and/or production (Reproduction, Male sterility), Resistance to herbicides (Glufosinate)
Characteristics of the modification process
pTTM8RE derived from pGV2260
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14973-6 Barnase | Bacillus amyloliquefaciens (BACAM)
    Protein coding sequence | Changes in physiology and/or production (Reproduction, Male sterility)
  • BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-103851-5 rbcS Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-101902-4 rbcS Transit Peptide | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101407-6 pTA29 pollen specific promoter | Nicotiana tabacum (Tobacco, TOBAC )
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
Chicory male-sterility system displaying glufosinate herbicide tolerance. Contains the barnase gene from Bacillus amyloliquefaciens and the bar gene encoding phosphinothricin N-acetyltransferase from Streptomyces hygroscopicus to confer tolerance to the herbicide phosphinothricin (Glufosinate ammonium).
EN
LMO characteristics
EN
  • Food
Detection method(s)
EN
Additional Information
Radicchio Rosso lines RM3-3, RM3-4, and RM3-6 (male sterile chicory plants) contain a ribonuclease encoded by barnase gene. The ribonuclease, which is expressed only in the tapetum cells of the pollen sac during anther development, blocks pollen development, thus producing a male sterile plant. To direct the expression of barnase to pollen cells, a pollen specific promoter, pTA 29 was used to direct the synthesis of ribonuclease enzyme.

Expression of barnase in pollen cells apparently results in degradation of host RNAs and blocking development of mature viable pollen. The barnase gene encodes for an extracellular ribonuclease protein in Bacillus amyloliquefaciens, a common soil microorganism. B. amyloliquefaciens also contains an intracellular protein called barstar which specifically inhibits barnase by combining with it in a one-to-one complex. Thus, barstar is produced intracellularly by the same organism that secretes barnase.

The second gene (bar) inserted into the Radicchio Rosso lines RM3-3, RM3-4, and RM3-6 genome encodes for phosphinothricin acetyltransferase (PAT) enzyme which inactivates phosphinothricin, the active component in the herbicide glufosinate. The bar gene is produced in the lines under the direction of Arabidospsis thaliana PssuAra promoter and nopaline synthase termination/polyadenylation sequences. Although these lines are tolerant to the herbicide, hybrid progeny (the seeds that would be available to the farmers) developed from these lines may or may not be tolerant to the herbicide.
EN
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