SYN-ØØØDA-9 - Tomato Modified for delayed softening | BCH-LMO-SCBD-15406 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 15 May 2013
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Tomato Modified for delayed softening
87-22/08 (Da)
Tomato with delayed softening through sense suppression of polygalacturonase (PG) enzyme activity from insertion of the a partial polygalacturonase (pg) gene, a pectin degrading enzyme derived from tomato.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
T7 line
  • SYN-ØØØØF-1 - Tomato Modified for delayed softening
    | Changes in physiology and/or production (Ripening), Resistance to antibiotics (Kanamycin)
  • SYN-ØØØØB-6 - Tomato Modified for delayed softening
    | Changes in physiology and/or production (Ripening), Resistance to antibiotics (Kanamycin)
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-15015-6 Polygalacturonase gene | Solanum lycopersicum (Tomato, SOLLC)
    Protein coding sequence | Changes in physiology and/or production (Ripening)
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
Southern blot and PCR analysis indicated that a single intact copy of the T-DNA was inserted into eh host genome
LMO characteristics
Detection method(s)
Additional Information
Reduced PG expression decreases the breakdown of pectin and leads to fruit with slowed cell wall breakdown, better viscosity characteristics and delayed softening. Tomato lines B, Da and F have improved harvest and processing properties that allow the transgenic tomatoes to remain longer on the vine to develop their natural flavour, maintain their firmness for shipping and produce a thicker consistency in processing.