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Living Modified Organism (LMO)
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Tomato Modified for delayed softeningEN
Tomato with delayed softening through sense suppression of polygalacturonase (PG) enzyme activity from insertion of the a partial polygalacturonase (pg) gene, a pectin degrading enzyme derived from tomato.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | (Bacteria)Protein coding sequence | Resistance to antibiotics (Kanamycin)
BCH-GENE-SCBD-15015-6 Polygalacturonase gene | (Tomato)Protein coding sequence | Changes in physiology and/or production (Ripening)
BCH-GENE-SCBD-100287-7 CaMV 35S promoterPromoter
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene TerminatorTerminator
BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene PromoterPromoter
Reduced PG expression decreases the breakdown of pectin and leads to fruit with slowed cell wall breakdown, better viscosity characteristics and delayed softening. Tomato lines B, Da and F have improved harvest and processing properties that allow the transgenic tomatoes to remain longer on the vine to develop their natural flavour, maintain their firmness for shipping and produce a thicker consistency in processing.