Herbicide tolerant maize | BCH-LMO-SCBD-258928 | Living Modified Organism | Biosafety Clearing-House

Loading...
Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
last updated: 19 Jan 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant maize
EN
DBN9858
No
The maize (Zea mays) was modified for tolerance to herbicides. For tolerance to glyphosate, the maize expresses Agrobacterium tumefaciens  5-enolpyruvylshikimate-3-phosphate synthase, which binds the glyphosate with low affinity and allows for the continued function of the shikimate pathway. For tolerance to glufosinate, the maize expresses  Streptomyces viridochromogenes phosphinothricin acetyltransferase, which inactivates the glufosinate ammonium herbicides through acetylation. 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
DBN10006
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)
    Plasmid vector
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)
    Promoter
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
The modified maize expresses two gene cassettes:  Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat) and Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps).

The pat coding sequence is under control of the a Cauliflower mosaic virus 35S promoter and terminator. High levels of expression are expected due to the nature of the promoter.

The cp4-epsps sequence is under control of an Oryza sativa actin 1 promoter and A. tumefaciens  nopaline synthase terminator. An Arabidopsis thaliana chloroplast transit peptide 2 sequence was included between the promoter and pat sequence to target the PAT protein to the chloroplast. High levels of expression in all tissues are expected due to the constitutive nature of the promoter.
EN
LMO characteristics
EN
  • Feed
  • Food
Detection method(s)
EN
Additional Information
EN