Green fluorescent zebrafish | BCH-LMO-SCBD-260325 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 29 Apr 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Green fluorescent zebrafish
EN
TK-2-Green
The zebrafish (Danio rerio) was modified for green fluorescent coloration through the expression of Aequorea victori green fluorescent protein. The fluorescent zebrafish has a few different names depending on the variety of zebrafish used in the transformation and subsequent breeding; examples include "Green Fluorescent Fairy", "Platinum Green Leopard Fluorescent Fairy", "Yellow Fluorescent Fairy" and "Green Ribbon Fluorescent Fairy".
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pZβ-GFP-1-ITR
EN
  • Microinjection
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-259257-1 β actin promoter | Danio rerio (Zebra fish, leopard danio, zebra danio)
    Promoter
  • BCH-GENE-SCBD-45846-4 Green Fluorescent Protein gene | (Crystal jellyfish)
    Protein coding sequence | Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-114748-3 SV40 poly-adenylation signal | Macaca mulatta polyomavirus 1 (SV40, Simian vacuolating virus 40, simian virus 40, Rhesus macaque polyomavirus)
    Terminator
  • BCH-GENE-SCBD-260317-1 Inverted terminal repeats | Adeno-associated dependoparvovirus A (Adeno-associated virus 2)
    Inverted terminal repeats
The modified zebrafish is expresses Aequorea victoria green fluorescent protein (gfp). The coding sequence is under control of the zebrafish beta-actin promoter and Macaca mulatta polyomavirus 1 (SV40) terminator. The promoter is expected to promote high levels of transcription in all body tissues. The Adeno-associated virus inverted terminal repeats found at either side of the cassette were used for integrating the transgene sequences into the zebrafish genome. 

Note: 
- The donor species of Adeno-associated virus inverted terminal repeats was not specified in the patent document. Thus, Adeno-associated virus 2 was chosen as a placeholder due to its use in recombinant vectors until further information becomes available.
- There is a discrepancy between the patent documents and the website regarding the promoter (where it is the alpha or beta actin promoter). Thus, the beta-actin promoter was selected to be consistent with the commercial website.
EN
LMO characteristics
EN
  • Ornamental
Detection method(s)
EN
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