MON-877Ø5-6 × MON-877Ø8-9 - Herbicide tolerant, High oleic acid soybean | BCH-LMO-SCBD-260407 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 19 May 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide tolerant, High oleic acid soybean
MON87705 × MON87708
MON-877Ø5-6 × MON-877Ø8-9
The soybean (Glycine max) was produced through crossing two modified parental lines for herbicide tolerance and an altered fatty acid profile. To increase the levels of oleic acid in the seeds, the soybean expresses an RNA interference cassette that silences the expression of delta(12)-fatty acid dehydrogenase and palmitoyl acyl carrier protein thioesterase, which allows oleic acid to accumulate by preventing the synthesis of palmitic acid, stearic acid and linolic acid from oleic acid. For tolerance to glyphosate, the soybean expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which allows for the continued functioning of the shikimate pathway (for aromatic amino acid synthesis). For tolerance to dicamba (3,6-dichloro-2-methoxybenzoic acid), the soybean expresses Stenotrophomonas maltophilia Dicamba monooxygenase, which catalyzes the oxidative demethylation of dicamba.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-LMO-SCBD-104683-5 Living Modified Organism MON-877Ø5-6 - Vistive Gold™ Soybean
    Changes in quality and/or metabolite content - Lipid and fatty acids Resistance to herbicides - Glyphosate
  • BCH-LMO-SCBD-104665-6 Living Modified Organism MON-877Ø8-9 - Dicamba Tolerant Soybean
    Resistance to herbicides
  • BCH-ORGA-SCBD-10453-6 Organism Glycine max (Soybean, Soya bean, Soya, SOYBN)
Characteristics of the modification process
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
DNA insert from MON88705 vector PV-GMPQ/HT4404
The MON87705 genome contains two gene cassettes: Agrobacteirum tumfaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) and an RNA interference (RNAi) cassette targeting endogenous delta(12)-fatty acid dehydrogenase (fad2-1) and palmitoyl acyl carrier protein thioesterase (fatb1-a) genes.

The epsps coding sequence is under control of an Arabidopsis thaliana elongator factor EF-1 alpha (EF-1α)-Figwort Mosaic Virus enhancer chimeric promoter and an Pisum sativum ribulose-1,5-bisphosphate carboxylase small subunit (rbcS-E9) terminator. An EF-1α leader and intron were also included to enhancer expression of epsps. An A. thaliana chloroplast transit peptide 2 sequence was also included to target the EPSPS protein to the chloroplast following translation.

The fad2-1 and fat1b-a RNAi cassette is under control of a Glycine max alpha subunit of the beta-conglycinin promoter and rbcS-E9 terminator. The sequence of the fad2-1 and fat1b-a sequences are arranged in an inverted repeat (fad2-1 (sense)-fat1b-a (sense)-fat1b-a (anti-sense)-fad2-1 (anti-sense)), such that upon transcription, the sense and anti-sense sequences base pair to form a hairpin structure (hairpin RNA), which will then trigger an RNAi response. Due to this response, no protein is expected to be translated. For more information, kindly refer to the 'Other gene(s) whose expression was affected by the transformation' section below. The promoter is expected to restrict expression of the RNAi cassette to seed tissues.

  • The promoter for the epsps cassette is a chimeric promoter consisting of the EF-1α promoter and an enhancer from Figwort Mosaic Virus. The chimeric promoter is 1040 basepairs in size and this size has been reflected on the EF-1α promoter of the genetic elements construct above.
  • The epsps sequence was codon optimized for expression in plants.
  • The parental genome of contains two T-DNA insertions:
    • T-DNA I contains epsps and a partial RNAi cassette (sense fragments of fad2-1 and fat1b-a).
    • T-DNA II contains the anti-sense fragments of fad2-1 and fat1b-a, as well as the terminator. 
  • The two T-DNAs were integrate a single locus to create a DNA insertion with a single epsps and RNAi cassette.

DNA insert from MON87708 vector PV-GMHT4355
The MON87708 genome contains a single gene cassette: Stenotrophomonas maltophilia Dicamba monooxygenase (dmo). The dmo sequence is under control of a Peanut chlorotic streak virus promoter and rbcS-E9 terminator. A Tobacco etch virus 5' untranslated region was included to enhance transcription of dmo. A P. sativum ribulose-1,5-bisphosphate carboxylase transit peptide was also included to direct the DMO protein to the chloroplast following translation.

  • Southern blot analysis indicated that a single dmo cassette was integrated into the MON87708 genome.

For more information, kindly refer to the parental LMO records.
LMO characteristics
  • BCH-GENE-SCBD-100267-2 delta(12)-fatty acid dehydrogenase | (Soybeans)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
  • BCH-GENE-SCBD-104682-2 Palmitoyl acyl carrier protein thioesterase 5'UTR | (Soybeans)
The RNAi cassette is arranged in the following order: fad2-1 (sense)-fat1b-a (sense)-fat1b-a (anti-sense)-fad2-1 (anti-sense). After transcription, the sense and anti-sense fragments will base pair due to complementarity, forming a hairpin RNA (hpRNA) with a partial double stranded structure. Due to the double strandedness of the hpRNA, an RNAi response will be triggered. The hpRNA will complex with DICER-like proteins to process the hpRNA into small interfering RNA (siRNA) of approximately 21-23 bases in length. These siRNAs will then be recruited by ARGONAUTE proteins to act as a guide to target mRNA molecules with complementary sequences for degradation. Thus, the fragments for fad2-1 and fat1b-a will target host transcripts and cause silencing of these endogenous genes. The silencing results in a differing fatty acid composition phenotype.
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