CTC-92Ø15-7 - Insect-resistant sugarcane | BCH-LMO-SCBD-260625 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
last updated: 14 Jun 2022
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant sugarcane
The sugarcane (Saccharum officinarum) event CTC-92015-7 expresses the Cry1Ac protein, which confers resistance to the sugarcane borer (Diatraea saccharalis), and the NPTII protein, used as a kanamycin selectable marker during transformation. This event uses sugarcane variety RB 92579 as the genetic background and thus differs from the other events developed by the Centro de Tecnologia Canavieira.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety RB 92579
  • CTC-95Ø19-5 - Insect-resistant sugarcane
    | Dr Wladecir Salles Oliveira | Resistance to antibiotics (Kanamycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Selectable marker genes and reporter genes
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
The modified sugarcane contains two gene cassettes: Bacillus thuringiensis crystal protein (cry1Ac) and Escherichia coli neomycin phosphotransferase II (nptII).

The cry1Ac coding sequence is under control of an enhanced Cauliflower mosaic virus 35S promoter and 35S terminator. The gene cassette additionally contains a Triticum aestivum 5' untranslated leader from chlorophyll a/b-binding protein and an Oryza sativa actin 1 intron for enhanced expression of cry1Ac. Due to the enhanced promoter and genetic elements, high levels of constitutive expression of Cry1Ac are expected.

The nptII coding sequence is under control of a Zea mays ubiquitin promoter and Agrobacterium tumefaciens nopaline synthase terminator. Expression of NPTII is expected in all plant tissues due to the nature of the ubiquitin promoter.

Note: The modified sugarcane event CTC-92015-7 expresses the same Cry1Ac and NPTII proteins, with 100% sequence homology, expressed by the reference CTC93209-4 modified sugarcane (see https://bch.cbd.int/en/database/record?documentID=115563).
LMO characteristics
  • Food
Detection method(s)
Additional Information
For more information on the genetic elements, see the pCTC146 vector map in the attached Chinese patent.